PMID- 16905536
OWN - NLM
STAT- MEDLINE
DCOM- 20061127
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 281
IP  - 41
DP  - 2006 Oct 13
TI  - Spectral and kinetic equivalence of oxidized cytochrome C oxidase as isolated and 
      "activated" by reoxidation.
PG  - 30319-25
AB  - The spectral and kinetic characteristics of two oxidized states of bovine heart 
      cytochrome c oxidase (CcO) have been compared. The first is the oxidized state of 
      enzyme isolated in the fast form (O) and the second is the form that is obtained 
      immediately after oxidation of fully reduced CcO with O2 (OH). No observable 
      differences were found between O and OH states in: (i) the rate of anaerobic 
      reduction of heme a3 for both the detergent-solubilized enzyme and for enzyme 
      embedded in its natural membraneous environment, (ii) the one-electron 
      distribution between heme a3 and CuB in the course of the full anaerobic 
      reduction, (iii) the optical and (iv) EPR spectra. Within experimental error of 
      these characteristics both forms are identical. Based on these observations it is 
      concluded that the reduction potentials and the ligation states of heme a3 and 
      CuB are the same for CcO in the O and OH states.
FAU - Jancura, Daniel
AU  - Jancura D
AD  - Department of Biophysics, Safarik University, Jesenna 5, 04154 Kosice, Slovak 
      Republic.
FAU - Berka, Vladimir
AU  - Berka V
FAU - Antalik, Marian
AU  - Antalik M
FAU - Bagelova, Jaroslava
AU  - Bagelova J
FAU - Gennis, Robert B
AU  - Gennis RB
FAU - Palmer, Graham
AU  - Palmer G
FAU - Fabian, Marian
AU  - Fabian M
LA  - eng
GR  - R01 HL016101/HL/NHLBI NIH HHS/United States
GR  - GM55807/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20060811
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 42VZT0U6YR (Heme)
RN  - 789U1901C5 (Copper)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Copper/chemistry
MH  - Electron Spin Resonance Spectroscopy
MH  - Electron Transport
MH  - Electron Transport Complex IV/*chemistry/metabolism
MH  - Electrons
MH  - Heme/chemistry
MH  - Kinetics
MH  - Models, Chemical
MH  - Myocardium/metabolism
MH  - Oxygen/*chemistry/metabolism
EDAT- 2006/08/15 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/08/15 09:00
PHST- 2006/08/15 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/08/15 09:00 [entrez]
AID - S0021-9258(19)89243-8 [pii]
AID - 10.1074/jbc.M605955200 [doi]
PST - ppublish
SO  - J Biol Chem. 2006 Oct 13;281(41):30319-25. doi: 10.1074/jbc.M605955200. Epub 2006 
      Aug 11.