PMID- 16906334
OWN - NLM
STAT- MEDLINE
DCOM- 20140815
LR  - 20060814
IS  - 0371-0874 (Print)
IS  - 0371-0874 (Linking)
VI  - 58
IP  - 4
DP  - 2006 Aug 25
TI  - Gene profile for differentiation of vascular adventitial myofibroblasts.
PG  - 337-44
AB  - Our previous study demonstrated that TGF-beta1 could induce the differentiation 
      of vascular adventitial fibroblasts (AFs) to myofibroblasts (MFs). The aim of 
      this study was to identify the genes which might be responsible for the cell 
      phenotypic change using genechips. Cultured rat AFs were treated with TGF-beta1 
      (10 ng/ml) for 0 min, 5 min, 15 min, 2 h, 12 h and 24 h, respectively. Then the 
      cells were gathered to prepare total RNA. We examined TGF-beta1-induced gene 
      expression profiling using Affymetrix oligonucleotide microarrays and analyzed 
      data by GCOS1.2 software. Moreover, expressional similarity was measured by 
      hierarchical clustering. Some of genechip results were confirmed by real-time 
      quantitative RT-PCR. Microarray analysis identified 2121 genes with a 2-fold 
      change or above after TGF-beta1 stimulation. 1318 genes showed a greater than 
      2-fold increase and 761 genes were reduced 2 folds or more at mRNA levels, 
      whereas a small portion of the total regulated genes (42 genes) displayed 
      dynamically up- and down-regulated pattern. Genes were further segregated for 
      early (peak at 5 min, 15 min and/or 2 h), late (peak at 12 h and/or 24 h), and 
      sustained (2-fold change or above at five time points) temporal response groups 
      according to the time of their peak expression level. Among 1318 up-regulated 
      genes, 333 genes (25.3%) responded rapidly to TGF-beta1 and 159 genes (12.1%) 
      responded in a sustained manner. Most genes (826, 62.6%) were regulated at 12 h 
      or later. For the 761 down-regulated genes, numbers of early and late responsive 
      genes were 335 (44%) and 267 (36.1%), respectively. There were also 159 genes, 
      19.9% of total down-regulated genes, decreased at five time points treated by 
      TGF-beta1. The results suggested that the gene expressions of secreted 
      phosphoprotein 1 (APP1) and Rho-associated coiled-coil forming kinase 2 (ROCK2) 
      had the same trends as alpha-smooth muscle-actin, a marker of MF differentiation. 
      In addition, the gene expression of potassium voltage-gated channel, Shal-related 
      family and member 2 (KCND2) was up-regulated. Furthermore, it was found that 
      endothelin 1 (EDN1), some complement components, NADPH oxidase 4 (NOX4) and 
      NAD(P)H dehydrogenase, quinone 1 (NQO1) might be involved in MF differentiation. 
      Using microarrary technique, we confirmed some genes that have been identified by 
      other techniques were implicated in MF differentiation and observed new genes 
      involved in this process. Our results suggest that gene expression profiling 
      study is helpful in identifying genes and pathways potentially involved in cell 
      differentiation.
FAU - Guo, Shu-Jie
AU  - Guo SJ
AD  - Shanghai Institute of Hypertension, Shanghai Jiaotong University School of 
      Medicine, Shanghai, China.
FAU - Wu, Ling-Yun
AU  - Wu LY
FAU - Shen, Wei-Li
AU  - Shen WL
FAU - Chen, Wen-Dong
AU  - Chen WD
FAU - Wei, Jian
AU  - Wei J
FAU - Gao, Ping-Jin
AU  - Gao PJ
FAU - Zhu, Ding-Liang
AU  - Zhu DL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Li Xue Bao
JT  - Sheng li xue bao : [Acta physiologica Sinica]
JID - 20730130R
SB  - IM
MH  - Adventitia/cytology
MH  - Animals
MH  - Aorta, Thoracic/*cytology
MH  - Cell Transdifferentiation/*genetics
MH  - Cells, Cultured
MH  - Female
MH  - Fibroblasts/*cytology
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - Male
MH  - Myofibroblasts/*cytology
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2006/08/15 09:00
MHDA- 2014/08/16 06:00
CRDT- 2006/08/15 09:00
PHST- 2006/08/15 09:00 [pubmed]
PHST- 2014/08/16 06:00 [medline]
PHST- 2006/08/15 09:00 [entrez]
PST - ppublish
SO  - Sheng Li Xue Bao. 2006 Aug 25;58(4):337-44.