PMID- 16906406
OWN - NLM
STAT- MEDLINE
DCOM- 20070309
LR  - 20131121
IS  - 0302-8933 (Print)
IS  - 0302-8933 (Linking)
VI  - 186
IP  - 4
DP  - 2006 Oct
TI  - Grouping of phenol hydroxylase and catechol 2,3-dioxygenase genes among phenol- 
      and p-cresol-degrading Pseudomonas species and biotypes.
PG  - 287-96
AB  - Phenol- and p-cresol-degrading pseudomonads isolated from phenol-polluted water 
      were analysed by the sequences of a large subunit of multicomponent phenol 
      hydroxylase (LmPH) and catechol 2,3-dioxygenase (C23O), as well as according to 
      the structure of the plasmid-borne pheBA operon encoding catechol 1,2-dioxygenase 
      and single component phenol hydoxylase. Comparison of the carA gene sequences 
      (encodes the small subunit of carbamoylphosphate synthase) between the strains 
      showed species- and biotype-specific phylogenetic grouping. LmPHs and C23Os 
      clustered similarly in P. fluorescens biotype B, whereas in P. mendocina strains 
      strong genetic heterogeneity became evident. P. fluorescens strains from biotypes 
      C and F were shown to possess the pheBA operon, which was also detected in the 
      majority of P. putida biotype B strains which use the ortho pathway for phenol 
      degradation. Six strains forming a separate LmPH cluster were described as the 
      first pseudomonads possessing the Mop type LmPHs. Two strains of this cluster 
      possessed the genes for both single and multicomponent PHs, and two had genetic 
      rearrangements in the pheBA operon leading to the deletion of the pheA gene. Our 
      data suggest that few central routes for the degradation of phenolic compounds 
      may emerge in bacteria as a result of the combination of genetically diverse 
      catabolic genes.
FAU - Merimaa, Merike
AU  - Merimaa M
AD  - Department of Genetics, Institute of Molecular and Cell Biology, University of 
      Tartu, Riia 23, 51010 Tartu, Estonia. merike.merimaa@ut.ee
FAU - Heinaru, Eeva
AU  - Heinaru E
FAU - Liivak, Merit
AU  - Liivak M
FAU - Vedler, Eve
AU  - Vedler E
FAU - Heinaru, Ain
AU  - Heinaru A
LA  - eng
SI  - GENBANK/AA064305
SI  - GENBANK/AB024741
SI  - GENBANK/AB031996
SI  - GENBANK/AF026065
SI  - GENBANK/AF228365
SI  - GENBANK/AF228366
SI  - GENBANK/AF228367
SI  - GENBANK/AF232713
SI  - GENBANK/AJ414216
SI  - GENBANK/AJ414217
SI  - GENBANK/AJ414218
SI  - GENBANK/AJ414222
SI  - GENBANK/AJ414224
SI  - GENBANK/AJ564846
SI  - GENBANK/AY538264
SI  - GENBANK/AY918068
SI  - GENBANK/AY973266
SI  - GENBANK/AY973267
SI  - GENBANK/D28864
SI  - GENBANK/DQ178181
SI  - GENBANK/DQ178182
SI  - GENBANK/DQ178183
SI  - GENBANK/DQ178184
SI  - GENBANK/DQ178185
SI  - GENBANK/DQ178186
SI  - GENBANK/DQ178187
SI  - GENBANK/DQ178188
SI  - GENBANK/DQ178189
SI  - GENBANK/DQ178190
SI  - GENBANK/DQ178191
SI  - GENBANK/DQ178192
SI  - GENBANK/DQ178193
SI  - GENBANK/DQ178194
SI  - GENBANK/DQ178195
SI  - GENBANK/DQ178196
SI  - GENBANK/DQ178197
SI  - GENBANK/DQ178198
SI  - GENBANK/DQ178199
SI  - GENBANK/DQ178200
SI  - GENBANK/DQ178201
SI  - GENBANK/DQ178202
SI  - GENBANK/DQ178203
SI  - GENBANK/DQ178204
SI  - GENBANK/DQ178205
SI  - GENBANK/DQ178206
SI  - GENBANK/DQ178207
SI  - GENBANK/DQ178208
SI  - GENBANK/DQ178209
SI  - GENBANK/DQ178210
SI  - GENBANK/DQ178211
SI  - GENBANK/DQ178212
SI  - GENBANK/DQ178213
SI  - GENBANK/DQ178214
SI  - GENBANK/DQ178215
SI  - GENBANK/DQ178216
SI  - GENBANK/DQ178217
SI  - GENBANK/DQ178218
SI  - GENBANK/DQ178219
SI  - GENBANK/DQ178220
SI  - GENBANK/DQ178221
SI  - GENBANK/DQ178222
SI  - GENBANK/DQ178223
SI  - GENBANK/DQ178224
SI  - GENBANK/DQ178225
SI  - GENBANK/DQ178226
SI  - GENBANK/DQ178227
SI  - GENBANK/DQ178228
SI  - GENBANK/DQ178229
SI  - GENBANK/DQ178230
SI  - GENBANK/DQ178231
SI  - GENBANK/DQ178232
SI  - GENBANK/DQ178233
SI  - GENBANK/DQ178234
SI  - GENBANK/DQ178235
SI  - GENBANK/M33263
SI  - GENBANK/M60276
SI  - GENBANK/M65205
SI  - GENBANK/P08127
SI  - GENBANK/V01161
SI  - GENBANK/X60740
SI  - GENBANK/X77856
SI  - GENBANK/X79063
SI  - GENBANK/X80765
SI  - GENBANK/Z36909
SI  - RefSeq/NP_943120
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060812
PL  - Germany
TA  - Arch Microbiol
JT  - Archives of microbiology
JID - 0410427
RN  - 0 (Cresols)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 1MXY2UM8NV (4-cresol)
RN  - 339NCG44TV (Phenol)
RN  - EC 1.- (Mixed Function Oxygenases)
RN  - EC 1.13.11.2 (Catechol 2,3-Dioxygenase)
RN  - EC 1.14.13.7 (phenol 2-monooxygenase)
RN  - EC 6.3.4.16 (Carbamoyl-Phosphate Synthase (Ammonia))
SB  - IM
MH  - Biodegradation, Environmental
MH  - Carbamoyl-Phosphate Synthase (Ammonia)/genetics
MH  - Catechol 2,3-Dioxygenase/*genetics/metabolism
MH  - Cresols/*metabolism
MH  - Mixed Function Oxygenases/*genetics/metabolism
MH  - Molecular Sequence Data
MH  - Phenol/*metabolism
MH  - Phylogeny
MH  - Pseudomonas/*classification/*enzymology/genetics/isolation & purification
MH  - RNA, Ribosomal, 16S/genetics
MH  - Rivers/microbiology
MH  - Sequence Analysis, DNA
MH  - Water Pollution, Chemical
EDAT- 2006/08/15 09:00
MHDA- 2007/03/10 09:00
CRDT- 2006/08/15 09:00
PHST- 2006/02/23 00:00 [received]
PHST- 2006/06/26 00:00 [accepted]
PHST- 2006/06/20 00:00 [revised]
PHST- 2006/08/15 09:00 [pubmed]
PHST- 2007/03/10 09:00 [medline]
PHST- 2006/08/15 09:00 [entrez]
AID - 10.1007/s00203-006-0143-3 [doi]
PST - ppublish
SO  - Arch Microbiol. 2006 Oct;186(4):287-96. doi: 10.1007/s00203-006-0143-3. Epub 2006 
      Aug 12.