PMID- 16908084
OWN - NLM
STAT- MEDLINE
DCOM- 20070404
LR  - 20171213
IS  - 0168-8227 (Print)
IS  - 0168-8227 (Linking)
VI  - 75
IP  - 3
DP  - 2007 Mar
TI  - Monocyte-derived macrophages from men and women with Type 2 diabetes mellitus 
      differ in fatty acid composition compared with non-diabetic controls.
PG  - 292-300
AB  - We examined whether macrophages from men and women with Type 2 diabetes mellitus 
      (T2DM) exhibited differences in expression of key genes involved in fatty acid 
      metabolism and in fatty acid composition compared with macrophages from 
      non-diabetic controls. Peripheral blood monocytes from subjects with T2DM (n=9) 
      and non-diabetic controls (n=10) were differentiated into macrophages in 10% 
      autologous serum and normal (5mM) or high (22mM) glucose. Levels of PPARalpha, 
      PPARgamma, LXRalpha, SCD and ABCA1 mRNAs were similar in macrophages from 
      subjects with T2DM and controls. At 5mM glucose, macrophage stearic acid (C18:0) 
      was 12.6+/-1.0% of total fatty acids for T2DM compared with 18.1+/-2.0% for 
      controls (p=0.03). Macrophage linoleic acid (C18:2) was 15.5+/-0.8% for T2DM and 
      9.3+/-2.0% for controls (p=0.005). The ratio of macrophage stearic acid 
      (C18:0)/oleic acid (C18:1) was 0.29 [0.25,0.48] for T2DM versus 0.54 [0.36,0.82] 
      for controls (p=0.04). Compared with non-diabetic controls, macrophages from men 
      and women with T2DM had significantly different fatty acid profiles consistent 
      with increased stearoyl-CoA desaturase (SCD) activity and increased C18:2 
      accumulation. This pattern of altered macrophage fatty acid composition may be 
      relevant to diabetic atherogenesis.
FAU - Senanayake, Saranga
AU  - Senanayake S
AD  - School of Medicine and Pharmacology, University of Western Australia, Fremantle 
      and Royal Perth Hospitals, Western Australia, Australia.
FAU - Brownrigg, Leon M
AU  - Brownrigg LM
FAU - Panicker, Vijay
AU  - Panicker V
FAU - Croft, Kevin D
AU  - Croft KD
FAU - Joyce, David A
AU  - Joyce DA
FAU - Steer, James H
AU  - Steer JH
FAU - Puddey, Ian B
AU  - Puddey IB
FAU - Yeap, Bu B
AU  - Yeap BB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060905
PL  - Ireland
TA  - Diabetes Res Clin Pract
JT  - Diabetes research and clinical practice
JID - 8508335
RN  - 0 (ABCA1 protein, human)
RN  - 0 (ATP Binding Cassette Transporter 1)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Fatty Acids)
RN  - 0 (Liver X Receptors)
RN  - 0 (NR1H3 protein, human)
RN  - 0 (Orphan Nuclear Receptors)
RN  - 0 (PPAR gamma)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (senescent cell differentiation antigen)
SB  - IM
MH  - ATP Binding Cassette Transporter 1
MH  - ATP-Binding Cassette Transporters/genetics
MH  - Adult
MH  - Antigens, Differentiation/genetics
MH  - DNA-Binding Proteins/genetics
MH  - Diabetes Mellitus, Type 2/*blood/genetics/physiopathology
MH  - Fatty Acids/*blood
MH  - Female
MH  - Humans
MH  - Liver X Receptors
MH  - Macrophages/*physiology
MH  - Male
MH  - Middle Aged
MH  - Monocytes/*cytology
MH  - Orphan Nuclear Receptors
MH  - PPAR gamma/genetics
MH  - RNA, Messenger/genetics/isolation & purification
MH  - Receptors, Cytoplasmic and Nuclear/genetics
MH  - Reference Values
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2006/08/16 09:00
MHDA- 2007/04/05 09:00
CRDT- 2006/08/16 09:00
PHST- 2006/06/08 00:00 [received]
PHST- 2006/07/10 00:00 [accepted]
PHST- 2006/08/16 09:00 [pubmed]
PHST- 2007/04/05 09:00 [medline]
PHST- 2006/08/16 09:00 [entrez]
AID - S0168-8227(06)00302-0 [pii]
AID - 10.1016/j.diabres.2006.07.009 [doi]
PST - ppublish
SO  - Diabetes Res Clin Pract. 2007 Mar;75(3):292-300. doi: 
      10.1016/j.diabres.2006.07.009. Epub 2006 Sep 5.