PMID- 16908224
OWN - NLM
STAT- MEDLINE
DCOM- 20070116
LR  - 20151119
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 844
IP  - 2
DP  - 2006 Dec 5
TI  - Simple, rapid and sensitive assay method for simultaneous quantification of 
      urinary nicotine and cotinine using gas chromatography-mass spectrometry.
PG  - 322-7
AB  - Nicotine is a major addictive compound in cigarette. Its smoke is rapidly and 
      extensively metabolized to several metabolites in human. Cotinine as a major 
      metabolite of nicotine is commonly used as a biomarker to determine active and 
      passive smokers. Cotinine has a longer half-life ( approximately 20 h) compared 
      to nicotine ( approximately 2h). A simple, sensitive, rapid and high throughput 
      GC-MS method was developed for simultaneous quantification of urinary nicotine 
      and cotinine in passive and active smokers. In the sample preparation method, the 
      analytes and internal standard were first basified and followed by liquid-liquid 
      extraction. Upon completion, anhydrous sodium sulphate was added to the solvent 
      mixture to trap moistures. The clear extract obtained was directly injected into 
      GC-MS, operating under selective ion monitoring (SIM) mode. Calibration curves in 
      the range of 0.5-5000 ng/mL of the analytes in urine matrix were established with 
      linear correlation coefficients (r(2)) greater than 0.997. The limit of detection 
      for both nicotine and cotinine were 0.20 ng/mL. The mean recoveries for nicotine 
      and cotinine were 93.0 and 100.4%, respectively. The within- and between-assay 
      accuracies were between 2.1 and 7.9% for nicotine and between 0.7 and 11.1% for 
      cotinine. Within- and between-assay precisions of 3.3-9.5% for nicotine and 
      3.4-9.8% for cotinine were also achieved. The method can be used in routine 
      assessment and monitoring of active smoking and exposure to environmental tobacco 
      smoke. The applicability of the assay was demonstrated in a small-scale 
      comparison study between smokers and non-smokers.
FAU - Man, Che Nin
AU  - Man CN
AD  - National Poison Centre, Universiti Sains Malaysia, Penang, Malaysia. 
      chenin@prn.usm.my <chenin@prn.usm.my>
FAU - Gam, Lay-Harn
AU  - Gam LH
FAU - Ismail, Syazwani
AU  - Ismail S
FAU - Lajis, Razak
AU  - Lajis R
FAU - Awang, Rahmat
AU  - Awang R
LA  - eng
PT  - Journal Article
DEP - 20060905
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 6M3C89ZY6R (Nicotine)
RN  - K5161X06LL (Cotinine)
SB  - IM
MH  - Adult
MH  - Cotinine/chemistry/*urine
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Humans
MH  - Male
MH  - Molecular Structure
MH  - Nicotine/chemistry/*urine
MH  - Reproducibility of Results
EDAT- 2006/08/16 09:00
MHDA- 2007/01/17 09:00
CRDT- 2006/08/16 09:00
PHST- 2006/06/19 00:00 [received]
PHST- 2006/07/07 00:00 [revised]
PHST- 2006/07/17 00:00 [accepted]
PHST- 2006/08/16 09:00 [pubmed]
PHST- 2007/01/17 09:00 [medline]
PHST- 2006/08/16 09:00 [entrez]
AID - S1570-0232(06)00589-7 [pii]
AID - 10.1016/j.jchromb.2006.07.029 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Dec 5;844(2):322-7. doi: 
      10.1016/j.jchromb.2006.07.029. Epub 2006 Sep 5.