PMID- 16908431
OWN - NLM
STAT- MEDLINE
DCOM- 20061101
LR  - 20240109
IS  - 1052-0295 (Print)
IS  - 1052-0295 (Linking)
VI  - 81
IP  - 2-3
DP  - 2006 Mar-Jun
TI  - Sensitive fluorescence in situ hybridization signal detection in maize using 
      directly labeled probes produced by high concentration DNA polymerase nick 
      translation.
PG  - 71-8
AB  - The signal produced by fluorescence in situ hybridization (FISH) often is 
      inconsistent among cells and sensitivity is low. Small DNA targets on the 
      chromatin are difficult to detect. We report here an improved nick translation 
      procedure for Texas red and Alexa Fluor 488 direct labeling of FISH probes. 
      Brighter probes can be obtained by adding excess DNA polymerase I. Using such 
      probes, a 30 kb yeast transgene, and the rp1, rp3 and zein multigene clusters 
      were clearly detected.
FAU - Kato, A
AU  - Kato A
AD  - Division of Biological Sciences, Tucker Hall, University of Missouri-Columbia, 
      Columbia, Missouri, 65211-7400, USA.
FAU - Albert, P S
AU  - Albert PS
FAU - Vega, J M
AU  - Vega JM
FAU - Birchler, J A
AU  - Birchler JA
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Biotech Histochem
JT  - Biotechnic & histochemistry : official publication of the Biological Stain 
      Commission
JID - 9107378
RN  - 0 (DNA Probes)
RN  - EC 2.7.7.7 (DNA Polymerase I)
SB  - IM
MH  - *Chromosomes, Plant
MH  - *DNA Polymerase I
MH  - DNA Probes/*chemical synthesis
MH  - In Situ Hybridization, Fluorescence/instrumentation/*methods
MH  - *Molecular Probe Techniques/instrumentation
MH  - Zea mays/*genetics
EDAT- 2006/08/16 09:00
MHDA- 2006/11/02 09:00
CRDT- 2006/08/16 09:00
PHST- 2006/08/16 09:00 [pubmed]
PHST- 2006/11/02 09:00 [medline]
PHST- 2006/08/16 09:00 [entrez]
AID - MTU8086171831U07 [pii]
AID - 10.1080/10520290600643677 [doi]
PST - ppublish
SO  - Biotech Histochem. 2006 Mar-Jun;81(2-3):71-8. doi: 10.1080/10520290600643677.