PMID- 1690892
OWN - NLM
STAT- MEDLINE
DCOM- 19900504
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 87
IP  - 7
DP  - 1990 Apr
TI  - Exon skipping during splicing of albumin mRNA precursors in Nagase analbuminemic 
      rats.
PG  - 2652-6
AB  - Based on the observation that albumin transcripts accumulate in the liver nuclear 
      RNA fraction of Nagase analbuminemic rats (NAR), it was proposed [Esumi, H., 
      Takahashi, Y., Sato, S., Nagase, S. & Sugimura, T. (1983) Proc. Natl. Acad. Sci. 
      USA 80, 95-99] [corrected] that a 7-base-pair deletion at the splice donor site 
      of intron H-I of the albumin gene in these animals leads to impaired processing 
      of albumin pre-mRNA. To identify the specific splicing abnormality, we examined 
      the primary structure of cytoplasmic albumin mRNA across the junctions of exons 
      G-H-I by RNase protection mapping, Northern blot hybridization, Southern blot 
      analysis of polymerase chain reaction-amplified cDNA, and DNA sequencing. The 
      major albumin mRNA species in NAR showed precise deletion of exon H, suggesting 
      that this exon was skipped during albumin pre-mRNA processing. Since the intron 
      G-H splice donor and acceptor sites and exon H sequence are normal, the finding 
      of exon H skipping in NAR has important implications regarding the mechanism of 
      splice site selection. Moreover, the NAR model provides an excellent system to 
      study splicing in vivo in a higher animal.
FAU - Shalaby, F
AU  - Shalaby F
AD  - Marion Bessin Liver Research Center, Albert Einstein College of Medicine, Bronx, 
      NY 10461.
FAU - Shafritz, D A
AU  - Shafritz DA
LA  - eng
GR  - P30 DK-41296/DK/NIDDK NIH HHS/United States
GR  - R01 DK-17609/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA Probes)
RN  - 0 (RNA Precursors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Serum Albumin)
RN  - 24937-83-5 (Poly A)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
RN  - EC 3.1.- (Ribonucleases)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Blotting, Southern
MH  - DNA/genetics
MH  - DNA Probes
MH  - *Exons
MH  - Male
MH  - Molecular Sequence Data
MH  - *Mutation
MH  - Plasmids
MH  - Poly A/genetics
MH  - Polymerase Chain Reaction
MH  - RNA/genetics
MH  - RNA Precursors/*genetics
MH  - *RNA Splicing
MH  - RNA, Messenger
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Rats, Mutant Strains
MH  - Restriction Mapping
MH  - Ribonucleases
MH  - Serum Albumin/deficiency/*genetics
PMC - PMC53748
EDAT- 1990/04/01 00:00
MHDA- 1990/04/01 00:01
PMCR- 1990/10/01
CRDT- 1990/04/01 00:00
PHST- 1990/04/01 00:00 [pubmed]
PHST- 1990/04/01 00:01 [medline]
PHST- 1990/04/01 00:00 [entrez]
PHST- 1990/10/01 00:00 [pmc-release]
AID - 10.1073/pnas.87.7.2652 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1990 Apr;87(7):2652-6. doi: 10.1073/pnas.87.7.2652.