PMID- 16910871
OWN - NLM
STAT- MEDLINE
DCOM- 20081118
LR  - 20211119
IS  - 1557-9077 (Electronic)
IS  - 1050-7256 (Linking)
VI  - 16
IP  - 8
DP  - 2006 Aug
TI  - Cloning and characterization of a novel isoform of iodotyrosine dehalogenase 1 
      (DEHAL1) DEHAL1C from human thyroid: comparisons with DEHAL1 and DEHAL1B.
PG  - 715-24
AB  - The human iodotyrosine dehalogenase 1 (DEHAL1) gene is composed of six exons. Two 
      isoforms (DEHAL1 and DEHAL1B) have been published in GenBank, both of which have 
      a nitroreductase domain and arise from differential splicing in exon 5. We 
      recently showed that the DEHAL1 isoform is a transmembrane protein that 
      efficiently catalyzes the NADPH-dependent deiodination of mono (L-MIT) and 
      diiodotyrosine (L-DIT) in human embryonic kidney-293 (HEK293) cells. In the 
      present study, we establish the existence of a new transcript, DEHAL1C, in the 
      human thyroid with a terminal exon that lacks in the DEHAL1 transcript. This exon 
      is the complete exon 5, which is spliced in the DEHAL1B mRNA variant. These two 
      variants encode proteins with differing C-terminal domains. Using quantitative 
      reverse transcription polymerase chain reaction, we found that the expression of 
      the mRNA of DEHAL1C and DEHAL1B was lower than that of DEHAL1 mRNA in the 
      thyroid. We also observed that human DEHAL1B and DEHAL1C proteins are rapidly 
      degraded in stably transfected HEK293 cells, unlike the DEHAL1 protein, and that 
      exposure to the proteasome inhibitor MG132 resulted in accumulation of these 
      proteins that was markedly time- and concentration-dependent. These findings show 
      that the cytoplasmic tail could play a role in the stability of the protein.
FAU - Gnidehou, Sedami
AU  - Gnidehou S
AD  - FRE 2939 CNRS, Institut Gustave Roussy, Villejuif, France., Laboratoire de 
      Pharmacologie et d'Hormonologie, ISBA, Cotonou, Benin, France.
FAU - Lacroix, Ludovic
AU  - Lacroix L
FAU - Sezan, Alphonse
AU  - Sezan A
FAU - Ohayon, Renee
AU  - Ohayon R
FAU - Noel-Hudson, Marie-Sophie
AU  - Noel-Hudson MS
FAU - Morand, Stanislas
AU  - Morand S
FAU - Francon, Jacques
AU  - Francon J
FAU - Courtin, Francoise
AU  - Courtin F
FAU - Virion, Alain
AU  - Virion A
FAU - Dupuy, Corinne
AU  - Dupuy C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Thyroid
JT  - Thyroid : official journal of the American Thyroid Association
JID - 9104317
RN  - 0 (Leupeptins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - EC 3.- (Hydrolases)
RN  - EC 3.8.1.2 (IYD protein, human)
RN  - RF1P63GW3K (benzyloxycarbonylleucyl-leucyl-leucine aldehyde)
SB  - IM
MH  - Alternative Splicing
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Dose-Response Relationship, Drug
MH  - Exons
MH  - Humans
MH  - Hydrolases/*chemistry/*genetics
MH  - Leupeptins/pharmacology
MH  - Membrane Proteins/*chemistry/*genetics
MH  - Models, Biological
MH  - Models, Genetic
MH  - *Protein Isoforms
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Thyroid Gland/*metabolism
MH  - Time Factors
EDAT- 2006/08/17 09:00
MHDA- 2008/11/19 09:00
CRDT- 2006/08/17 09:00
PHST- 2006/08/17 09:00 [pubmed]
PHST- 2008/11/19 09:00 [medline]
PHST- 2006/08/17 09:00 [entrez]
AID - 10.1089/thy.2006.16.715 [doi]
PST - ppublish
SO  - Thyroid. 2006 Aug;16(8):715-24. doi: 10.1089/thy.2006.16.715.