PMID- 16911712
OWN - NLM
STAT- MEDLINE
DCOM- 20061017
LR  - 20111117
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 56
IP  - 3
DP  - 2006 Sep
TI  - Human leukocyte antigen F protein is expressed in the extra-villous trophoblasts 
      but not on the cell surface of them.
PG  - 172-7
AB  - PROBLEM: Human leukocyte antigen (HLA)-F together with other class 1b HLAs may 
      play a pivotal role in immunological interaction at feto-maternal interface. 
      However, it is not elucidated whether HLA-F is expressed on cell surface of 
      extra-villous trophoblasts (EVTs). METHOD OF STUDY: The localization of HLA-F in 
      placenta of each trimester was investigated by immunohistochemistry. The 
      expression modality of HLA-F molecule was compared with that of HLA-G in cultured 
      EVTs using flowcytometry. RESULTS AND CONCLUSION: In immunohistochemical study, 
      remarkable staining for HLA-F was confirmed in EVTs throughout gestation. HLA-F 
      expression was restricted to be intracellular in cultured EVTs. HLA-F expression 
      was increased with time in culture, which was accompanied by the increase of cell 
      surface HLA-G. Consequently, HLA-F lacking in cell surface expression on EVTs may 
      not contribute to the direct cell-to-cell immune reaction but might work 
      supportively modifying the function of HLA-G.
FAU - Nagamatsu, Takeshi
AU  - Nagamatsu T
AD  - Department of Obstetrics and Gynecology, Faculty of Medicine, University of 
      Tokyo, Tokyo, Japan.
FAU - Fujii, Tomoyuki
AU  - Fujii T
FAU - Matsumoto, Junko
AU  - Matsumoto J
FAU - Yamashita, Takahiro
AU  - Yamashita T
FAU - Kozuma, Shiro
AU  - Kozuma S
FAU - Taketani, Yuji
AU  - Taketani Y
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-F antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Cell Culture Techniques
MH  - Cell Line, Tumor
MH  - Cell Membrane/immunology
MH  - Female
MH  - Flow Cytometry
MH  - HLA Antigens/*analysis/metabolism
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/*analysis/metabolism
MH  - Humans
MH  - Pregnancy
MH  - Trophoblasts/cytology/*immunology/metabolism
EDAT- 2006/08/17 09:00
MHDA- 2006/10/18 09:00
CRDT- 2006/08/17 09:00
PHST- 2006/08/17 09:00 [pubmed]
PHST- 2006/10/18 09:00 [medline]
PHST- 2006/08/17 09:00 [entrez]
AID - AJI414 [pii]
AID - 10.1111/j.1600-0897.2006.00414.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 2006 Sep;56(3):172-7. doi: 10.1111/j.1600-0897.2006.00414.x.