PMID- 16911943
OWN - NLM
STAT- MEDLINE
DCOM- 20061219
LR  - 20141120
IS  - 1499-3872 (Print)
VI  - 5
IP  - 3
DP  - 2006 Aug
TI  - Effects of cytotoxic T lymphocytes on hepatoma cell line SMMC-7721 induced by 
      different subsets of dendritic cells in vitro.
PG  - 422-7
AB  - BACKGROUND: Dendritic cells (DCs) loaded with complex antigen are always used to 
      induce cytotoxic T lymphocytes (CTLs) which have a specific anti-tumor activity. 
      However, CTLs can assault autologous cells induced by DCs loaded with autologous 
      antigen. This study aimed to explore how to weaken the autoimmune reaction 
      induced by DC vaccine by combining mature DC (mDC) activating immunity and 
      immature DC (imDC) leading to immune tolerance to make hepatocellular carcinoma 
      (HCC) vaccine in vitro. METHODS: DC progenitors derived from human peripheral 
      blood were assigned to two groups. One was cultured to mDC and pulsed with 
      frozen-thawed antigen (FTA) of human HCC cell line SMMC-7721 cells (mDC group), 
      and the other was cultured to imDC and pulsed with FTA of human liver cell line 
      L-02 cells (imDC group). The morphology of DCs was monitored and cells phenotypes 
      including HLA-DR, CD80, CD1alpha, CD83 were assayed by flowcytometry (FCM). The 
      concentrations of interleukin-12 (IL-12) in the supernatant were assayed by 
      ELISA. Methyl thiazolyl tetrazolium (MTT) was used to evaluate T cell 
      proliferation induced by mDC and imDC and the killing rate of CTL induced by mDC 
      and imDC respectively/together on SMMC-7721 and L-02 cells. RESULTS: Compared 
      with the imDC group, the mDC group was characterized by the following: increased 
      secretion of IL-12 (P<0.05); higher expression of HLA-DR, CD1alpha, CD80, CD83; 
      and stronger activity in stimulating proliferation of isogenic T cells (P<0.05). 
      CTL induced by the mDC group had a significant killing response to SMMC-7721 as 
      well as a higher killing rate for L-02 (P>0.05). CTL induced by mDC and imDC 
      together had a higher killing response to SMMC-7721, but a lower killing rate for 
      L-02 (P<0.01). CONCLUSIONS: CTL induced by mDC and imDC together has a higher 
      antigen-specific killing response in vitro than that induced by mDC alone. This 
      may be of greater clinical value.
FAU - Wang, Jia-Xiang
AU  - Wang JX
AD  - Department of Surgery, First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou 450052, China. wjiaxiang@zzu.edu.cn
FAU - Liu, Guang-Hui
AU  - Liu GH
FAU - Fan, Ying-Zhong
AU  - Fan YZ
FAU - Liu, Qiu-Liang
AU  - Liu QL
FAU - Zhou, Juan
AU  - Zhou J
FAU - Zhang, Dong-Yun
AU  - Zhang DY
FAU - Qi, Yuan-Ming
AU  - Qi YM
LA  - eng
PT  - Journal Article
PL  - Singapore
TA  - Hepatobiliary Pancreat Dis Int
JT  - Hepatobiliary & pancreatic diseases international : HBPD INT
JID - 101151457
SB  - IM
MH  - Carcinoma, Hepatocellular/*immunology
MH  - Cell Line, Tumor
MH  - Dendritic Cells/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Flow Cytometry
MH  - Humans
MH  - In Vitro Techniques
MH  - T-Lymphocytes, Cytotoxic/*immunology
EDAT- 2006/08/17 09:00
MHDA- 2006/12/21 09:00
CRDT- 2006/08/17 09:00
PHST- 2006/08/17 09:00 [pubmed]
PHST- 2006/12/21 09:00 [medline]
PHST- 2006/08/17 09:00 [entrez]
AID - 868 [pii]
PST - ppublish
SO  - Hepatobiliary Pancreat Dis Int. 2006 Aug;5(3):422-7.