PMID- 16913916
OWN - NLM
STAT- MEDLINE
DCOM- 20070118
LR  - 20060817
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 8
IP  - 9
DP  - 2006 Sep
TI  - Phylogeny, physiology and distribution of 'Candidatus Microthrix calida', a new 
      Microthrix species isolated from industrial activated sludge wastewater treatment 
      plants.
PG  - 1552-63
AB  - Twelve strains of filamentous bacteria morphologically identified as 'Microthrix 
      parvicella' were isolated from industrial activated sludge wastewater treatment 
      plants. 16S rRNA gene sequences analysis showed that these strains were all 
      closely related to 'Candidatus Microthrix parvicella'. Six of them, however, had 
      a 16S rRNA gene similarity of only 95.7% and 96.7% to 'Candidatus Microthrix 
      parvicella' suggesting the presence of a new species. The name 'Candidatus 
      Microthrix calida' is proposed for this new microorganism. The physiological 
      properties of these six isolates supported the description of a new taxon. The 
      'Candidatus Microthrix calida' strains produced thin filaments (0.3-0.7 microm 
      diameter), they did not grow on the media supporting the growth of 'Candidatus 
      Microthrix parvicella' and could be cultivated at higher temperature (up to 36.5 
      degrees C). Preliminary data on substrate uptake were obtained by 
      microautoradiography on pure culture. Two new fluorescence in situ hybridization 
      (FISH) probes, Mpa-T1-1260 specific for 'Candidatus Microthrix calida' and 
      Mpa-all-1410 targeting both Microthrix species, were designed. The presence of 
      Microthrix spp. was investigated in 114 activated sludge plants. 'Microthrix 
      parvicella' morphotype was detected in 23% of the analysed samples and FISH 
      analysis revealed that 'Candidatus Microthrix calida' was present in 5% of them. 
      The remaining 'M. parvicella' filaments were positive with probe Mpa-all-1410 but 
      could not all be identified as 'Candidatus Microthrix parvicella' suggesting the 
      presence of more hitherto undescribed biodiversity within this morphotype.
FAU - Levantesi, Caterina
AU  - Levantesi C
AD  - CNR, Water Research Institute, Via Reno 1, 00198 Rome, Italy. 
      levantesi@irsa.cnr.it
FAU - Rossetti, Simona
AU  - Rossetti S
FAU - Thelen, Karin
AU  - Thelen K
FAU - Kragelund, Caroline
AU  - Kragelund C
FAU - Krooneman, Janneke
AU  - Krooneman J
FAU - Eikelboom, Dick
AU  - Eikelboom D
FAU - Nielsen, Per Halkjaer
AU  - Nielsen PH
FAU - Tandoi, Valter
AU  - Tandoi V
LA  - eng
SI  - GENBANK/DQ147277
SI  - GENBANK/DQ147278
SI  - GENBANK/DQ147279
SI  - GENBANK/DQ147280
SI  - GENBANK/DQ147281
SI  - GENBANK/DQ147282
SI  - GENBANK/DQ147283
SI  - GENBANK/DQ147284
SI  - GENBANK/DQ147285
SI  - GENBANK/DQ147286
SI  - GENBANK/DQ147287
SI  - GENBANK/DQ147288
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (Manure)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
SB  - IM
MH  - *Actinobacteria/classification/physiology
MH  - Animals
MH  - In Situ Hybridization, Fluorescence
MH  - Manure/*microbiology
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/classification
MH  - Sewage/*microbiology
EDAT- 2006/08/18 09:00
MHDA- 2007/01/19 09:00
CRDT- 2006/08/18 09:00
PHST- 2006/08/18 09:00 [pubmed]
PHST- 2007/01/19 09:00 [medline]
PHST- 2006/08/18 09:00 [entrez]
AID - EMI1046 [pii]
AID - 10.1111/j.1462-2920.2006.01046.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2006 Sep;8(9):1552-63. doi: 10.1111/j.1462-2920.2006.01046.x.