PMID- 16914428
OWN - NLM
STAT- MEDLINE
DCOM- 20060927
LR  - 20200930
IS  - 0363-6119 (Print)
IS  - 1522-1490 (Electronic)
IS  - 0363-6119 (Linking)
VI  - 291
IP  - 2
DP  - 2006 Aug
TI  - Identification of neural circuits involved in female genital responses in the 
      rat: a dual virus and anterograde tracing study.
PG  - R419-28
AB  - The spinal and peripheral innervation of the clitoris and vagina are fairly well 
      understood. However, little is known regarding supraspinal control of these 
      pelvic structures. The multisynaptic tracer pseudorabies virus (PRV) was used to 
      map the brain neurons that innervate the clitoris and vagina. To delineate 
      forebrain input on PRV-labeled cells, the anterograde tracer biotinylated dextran 
      amine was injected in the medial preoptic area (MPO), ventromedial nucleus of the 
      hypothalamus (VMN), or the midbrain periaqueductal gray (PAG) 10 days before 
      viral injections. These brain regions have been intimately linked to various 
      aspects of female reproductive behavior. After viral injections (4 days) in the 
      vagina and clitoris, PRV-labeled cells were observed in the paraventricular 
      nucleus (PVN), Barrington's nucleus, the A5 region, and the nucleus 
      paragigantocellularis (nPGi). At 5 days postviral administration, additional 
      PRV-labeled cells were observed within the preoptic region, VMN, PAG, and lateral 
      hypothalamus. Anterograde labeling from the MPO terminated among PRV-positive 
      cells primarily within the dorsal PVN of the hypothalamus, ventrolateral VMN 
      (VMNvl), caudal PAG, and nPGi. Anterograde labeling from the VMN terminated among 
      PRV-positive cells in the MPO and lateral/ventrolateral PAG. Anterograde labeling 
      from the PAG terminated among PRV-positive cells in the PVN, ventral 
      hypothalamus, and nPGi. Transynaptically labeled cells in the lateral 
      hypothalamus, Barrington's nucleus, and ventromedial medulla received innervation 
      from all three sources. These studies, together, identify several central nervous 
      system (CNS) sites participating in the neural control of female sexual 
      responses. They also provide the first data demonstrating a link between the MPO, 
      VMNvl, and PAG and CNS regions innervating the clitoris and vagina, providing 
      support that these areas play a major role in female genital responses.
FAU - Marson, L
AU  - Marson L
AD  - Division of Urology, Department of Surgery, School of Medicine, University of 
      North Carolina, Chapel Hill, NC, USA. lmarson@med.unc.edu
FAU - Murphy, A Z
AU  - Murphy AZ
LA  - eng
GR  - R01 NS039166/NS/NINDS NIH HHS/United States
GR  - R01 MH059187-04/MH/NIMH NIH HHS/United States
GR  - R01 DA016272/DA/NIDA NIH HHS/United States
GR  - DA-16272/DA/NIDA NIH HHS/United States
GR  - R01 MH059187-01/MH/NIMH NIH HHS/United States
GR  - R01 MH059187-02/MH/NIMH NIH HHS/United States
GR  - R01 MH059187-03/MH/NIMH NIH HHS/United States
GR  - NS-39166/NS/NINDS NIH HHS/United States
GR  - MH-59197/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20060216
PL  - United States
TA  - Am J Physiol Regul Integr Comp Physiol
JT  - American journal of physiology. Regulatory, integrative and comparative 
      physiology
JID - 100901230
RN  - 0 (Dextrans)
RN  - 0 (biotinylated dextran amine)
RN  - 6SO6U10H04 (Biotin)
SB  - IM
MH  - Afferent Pathways/physiology
MH  - Animals
MH  - Biotin/analogs & derivatives
MH  - Brain/*physiology
MH  - Brain Mapping/*methods
MH  - Clitoris/innervation
MH  - Dextrans
MH  - Female
MH  - Genitalia, Female/*innervation
MH  - Herpesvirus 1, Suid
MH  - Hypothalamus/physiology
MH  - Neural Pathways/physiology
MH  - Neurons/physiology
MH  - Preoptic Area/physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sexual Behavior, Animal
MH  - Spinal Cord/physiology
MH  - Vagina/innervation
PMC - PMC2836019
MID - NIHMS175637
EDAT- 2006/08/18 09:00
MHDA- 2006/09/28 09:00
PMCR- 2010/03/10
CRDT- 2006/08/18 09:00
PHST- 2006/08/18 09:00 [pubmed]
PHST- 2006/09/28 09:00 [medline]
PHST- 2006/08/18 09:00 [entrez]
PHST- 2010/03/10 00:00 [pmc-release]
AID - 00864.2005 [pii]
AID - 10.1152/ajpregu.00864.2005 [doi]
PST - ppublish
SO  - Am J Physiol Regul Integr Comp Physiol. 2006 Aug;291(2):R419-28. doi: 
      10.1152/ajpregu.00864.2005. Epub 2006 Feb 16.