PMID- 16915405
OWN - NLM
STAT- MEDLINE
DCOM- 20070423
LR  - 20181201
IS  - 0031-6768 (Print)
IS  - 0031-6768 (Linking)
VI  - 453
IP  - 1
DP  - 2006 Oct
TI  - Homing and differentiation of mesenchymal stem cells delivered intravenously to 
      ischemic myocardium in vivo: a time-series study.
PG  - 43-52
AB  - Mesenchymal stem cells (MSCs) are potential sources of cells for tissue repair. 
      However, little information is available about the time course of homing and 
      differentiation of systemically delivered MSCs after acute myocardial ischemia 
      (MI). In the present study, MSCs were isolated from male rat bone marrow and 
      expanded in vitro. Female rats were divided randomly into three groups. Three 
      hours after coronary ligation, the transplanted group received an infusion of 
      MSCs through the tail vein; at the same time, a coronary-ligated control group 
      was injected with culture medium, and a normal (unligated) group received MSCs. 
      Homing of MSCs to the heart was assessed by expression of the Y chromosome sry 
      gene using fluorescence in situ hybridization (FISH) at 3 days, 1, 4, and 8 weeks 
      after transplantation. Immunofluorescent staining was used to examine markers for 
      cardiomyocytes, endothelial cells, and smooth muscle cells. Hemodynamics in the 
      hearts was also measured to assess cardiac function. At each time point, 
      sry-positive cells were present in the cardiac tissue in transplanted group but 
      not in the hearts of normal and control group animals. The number of sry-positive 
      cells was significantly higher at 1 week compared to 3 days after 
      transplantation. No significant difference was found in the number of 
      sry-positive cells among those of 1, 4, and 8 weeks after transplantation. At 3 
      days and 1 week after transplantation, the sry-positive cells in the transplanted 
      group lacked troponin, desmin, smooth muscle alpha-actin, and CD31. At the later 
      time points, cardiomyocytes, smooth muscle cells, and endothelial cells bearing 
      sry were identified in the transplanted group. The cardiac function in 
      transplanted group showed higher improvement at 4 and 8 weeks compared to 1 week 
      after transplantation. Our data suggest that intravenously delivered MSCs are 
      capable of homing toward the ischemic myocardium, and the fastigium of homing 
      appeared around 1 week after MI. The differentiation of MSCs to cardiomyocytes, 
      smooth muscle cells, and endothelial cells shows to be time dependent and arises 
      at 1 to 4 weeks after transplantation.
FAU - Jiang, Wenhui
AU  - Jiang W
AD  - Medical College, Xi'an Jiaotong University, No. 1 Jiankang Road, Xi'an, Shaanxi 
      710061, China.
FAU - Ma, Aiqun
AU  - Ma A
FAU - Wang, Tingzhong
AU  - Wang T
FAU - Han, Ke
AU  - Han K
FAU - Liu, Yu
AU  - Liu Y
FAU - Zhang, Yanmin
AU  - Zhang Y
FAU - Dong, Anping
AU  - Dong A
FAU - Du, Yuan
AU  - Du Y
FAU - Huang, Xin
AU  - Huang X
FAU - Wang, Jun
AU  - Wang J
FAU - Lei, Xinjun
AU  - Lei X
FAU - Zheng, Xiaopu
AU  - Zheng X
LA  - eng
PT  - Journal Article
DEP - 20060817
PL  - Germany
TA  - Pflugers Arch
JT  - Pflugers Archiv : European journal of physiology
JID - 0154720
SB  - IM
MH  - Animals
MH  - Cell Differentiation/physiology
MH  - Female
MH  - Heart/*physiology
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/cytology/*physiology
MH  - Myocardial Ischemia/pathology/*therapy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Regeneration/*physiology
MH  - Time Factors
EDAT- 2006/08/18 09:00
MHDA- 2007/04/24 09:00
CRDT- 2006/08/18 09:00
PHST- 2006/04/27 00:00 [received]
PHST- 2006/06/08 00:00 [accepted]
PHST- 2006/05/29 00:00 [revised]
PHST- 2006/08/18 09:00 [pubmed]
PHST- 2007/04/24 09:00 [medline]
PHST- 2006/08/18 09:00 [entrez]
AID - 10.1007/s00424-006-0117-y [doi]
PST - ppublish
SO  - Pflugers Arch. 2006 Oct;453(1):43-52. doi: 10.1007/s00424-006-0117-y. Epub 2006 
      Aug 17.