PMID- 16917511
OWN - NLM
STAT- MEDLINE
DCOM- 20070105
LR  - 20230210
IS  - 0893-3952 (Print)
IS  - 0893-3952 (Linking)
VI  - 19
IP  - 11
DP  - 2006 Nov
TI  - MALT1 gene rearrangements and NF-kappaB activation involving p65 and p50 are 
      absent or rare in primary MALT lymphomas of the breast.
PG  - 1402-8
AB  - Mucosa associated lymphoid tissue (MALT) lymphomas arising in the breast are 
      uncommon and few cases have been assessed for MALT lymphoma-associated 
      translocations, BCL-10 expression, or NF-kappaB activation. In this study, we 
      analyzed eight cases of primary breast MALT lymphoma. We also included 14 cases 
      of primary breast diffuse large B-cell lymphoma since some of these may represent 
      transformation of MALT lymphoma, known to occur at extra-mammary MALT sites. All 
      cases were assessed for MALT1 gene rearrangements by fluorescence in situ 
      hybridization (FISH). Using immunohistochemical methods, all cases were assessed 
      for BCL-10, and subsets were assessed for NF-kappaB p65 and p50. None of the 
      cases had MALT1 gene rearrangements by FISH. Of eight MALT lymphomas, BCL-10 was 
      positive in seven (88%), with moderate nuclear and cytoplasmic staining in six, 
      and a weak cytoplasmic staining in one. NF-kappaB p65 (n=8) and p50 (n=5) were 
      negative or showed only cytoplasmic staining (ie inactivated) in all cases. Of 14 
      diffuse large B-cell lymphoma cases, BCL-10 was positive in 12 (87%), with 
      weak-to-moderate cytoplasmic staining in 10, weak cytoplasmic and focally nuclear 
      staining in one, and a moderate-to-strong nuclear and cytoplasmic staining in 
      one. NF-kappaB p65 (n=11) showed cytoplasmic staining in all cases, whereas p50 
      (n=8) showed nuclear positivity (ie activated) in two (25%) cases. We conclude 
      that MALT1 gene rearrangements are absent or rare in primary breast MALT lymphoma 
      and diffuse large B-cell lymphoma. In MALT lymphomas, the moderate BCL-10 nuclear 
      expression in six neoplasms is inconsistent with the FISH results, suggesting 
      that BCL-10 immunostaining overestimates the frequency of MALT1 gene 
      rearrangements. We also could not demonstrate NF-kappaB activation using nuclear 
      staining for p65 and p50. In contrast, breast diffuse large B-cell lymphomas are 
      heterogeneous. Weak cytoplasmic BCL-10 staining in most cases and evidence of 
      NF-kappaB p50 activation in a subset differs from breast MALT lymphomas.
FAU - Talwalkar, Sameer S
AU  - Talwalkar SS
AD  - Department of Hematopathology, The University of Texas MD Anderson Cancer Center, 
      Houston, TX 77030, USA.
FAU - Valbuena, Jose R
AU  - Valbuena JR
FAU - Abruzzo, Lynne V
AU  - Abruzzo LV
FAU - Admirand, Joan H
AU  - Admirand JH
FAU - Konoplev, Sergej N
AU  - Konoplev SN
FAU - Bueso-Ramos, Carlos E
AU  - Bueso-Ramos CE
FAU - Medeiros, L Jeffrey
AU  - Medeiros LJ
LA  - eng
PT  - Journal Article
DEP - 20060818
PL  - United States
TA  - Mod Pathol
JT  - Modern pathology : an official journal of the United States and Canadian Academy 
      of Pathology, Inc
JID - 8806605
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (B-Cell CLL-Lymphoma 10 Protein)
RN  - 0 (BCL10 protein, human)
RN  - 0 (NF-kappa B)
RN  - 0 (NF-kappa B p50 Subunit)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Transcription Factor RelA)
RN  - EC 3.4.22.- (Caspases)
RN  - EC 3.4.22.- (MALT1 protein, human)
RN  - EC 3.4.22.- (Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/analysis
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - B-Cell CLL-Lymphoma 10 Protein
MH  - Breast Neoplasms/chemistry/*genetics/*pathology
MH  - Breast Neoplasms, Male/genetics
MH  - Caspases/*genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - *Gene Rearrangement
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, B-Cell, Marginal Zone/chemistry/*genetics/*pathology
MH  - Lymphoma, Large B-Cell, Diffuse/genetics
MH  - Male
MH  - Middle Aged
MH  - Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein
MH  - NF-kappa B/*analysis
MH  - NF-kappa B p50 Subunit/analysis
MH  - Neoplasm Proteins/*genetics
MH  - Transcription Factor RelA/analysis
EDAT- 2006/08/19 09:00
MHDA- 2007/01/06 09:00
CRDT- 2006/08/19 09:00
PHST- 2006/08/19 09:00 [pubmed]
PHST- 2007/01/06 09:00 [medline]
PHST- 2006/08/19 09:00 [entrez]
AID - S0893-3952(22)02040-3 [pii]
AID - 10.1038/modpathol.3800668 [doi]
PST - ppublish
SO  - Mod Pathol. 2006 Nov;19(11):1402-8. doi: 10.1038/modpathol.3800668. Epub 2006 Aug 
      18.