PMID- 16919167
OWN - NLM
STAT- MEDLINE
DCOM- 20061120
LR  - 20181113
IS  - 1741-7007 (Electronic)
IS  - 1741-7007 (Linking)
VI  - 4
DP  - 2006 Aug 18
TI  - A molecular scheme for improved characterization of human embryonic stem cell 
      lines.
PG  - 28
AB  - BACKGROUND: Human embryonic stem cells (hESC) offer a renewable source of a wide 
      range of cell types for use in research and cell-based therapies to treat 
      disease. Inspection of protein markers provides important information about the 
      current state of the cells and data for subsequent manipulations. However, hESC 
      must be routinely analyzed at the genomic level to guard against deleterious 
      changes during extensive propagation, expansion, and manipulation in vitro. 
      RESULTS: We found that short tandem repeat (STR) analysis, human leukocyte 
      antigen (HLA) typing, single nucleotide polymorphism (SNP) genomic analysis, 
      mitochondrial DNA sequencing, and gene expression analysis by microarray can be 
      used to fully describe any hESC culture in terms of its identity, stability, and 
      undifferentiated state. CONCLUSION: Here we describe, using molecular biology 
      alone, a comprehensive characterization of 17 different hESC lines. The use of 
      amplified nucleic acids means that for the first time full characterization of 
      hESC lines can be performed with little time investment and a minimum of 
      material. The information thus gained will facilitate comparison of lines and 
      replication of results between laboratories.
FAU - Josephson, Richard
AU  - Josephson R
AD  - Stem Cell Center, American Type Culture Collection (ATCC), Manassas, VA, USA. 
      rjosephson@globalstem.com
FAU - Sykes, Gregory
AU  - Sykes G
FAU - Liu, Ying
AU  - Liu Y
FAU - Ording, Carol
AU  - Ording C
FAU - Xu, Weining
AU  - Xu W
FAU - Zeng, Xianmin
AU  - Zeng X
FAU - Shin, Soojung
AU  - Shin S
FAU - Loring, Jeanne
AU  - Loring J
FAU - Maitra, Anirban
AU  - Maitra A
FAU - Rao, Mahendra S
AU  - Rao MS
FAU - Auerbach, Jonathan M
AU  - Auerbach JM
LA  - eng
GR  - N01AG40002/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20060818
PL  - England
TA  - BMC Biol
JT  - BMC biology
JID - 101190720
RN  - 0 (Genetic Markers)
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Cell Differentiation
MH  - *Cell Line
MH  - Coculture Techniques
MH  - Embryonic Stem Cells/*cytology
MH  - Genetic Markers
MH  - HLA Antigens/genetics
MH  - Humans
MH  - Microsatellite Repeats
MH  - Oligonucleotide Array Sequence Analysis
MH  - Pluripotent Stem Cells/*cytology
MH  - Polymorphism, Single Nucleotide
PMC - PMC1601965
EDAT- 2006/08/22 09:00
MHDA- 2006/12/09 09:00
PMCR- 2006/08/18
CRDT- 2006/08/22 09:00
PHST- 2006/02/06 00:00 [received]
PHST- 2006/08/18 00:00 [accepted]
PHST- 2006/08/22 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/08/22 09:00 [entrez]
PHST- 2006/08/18 00:00 [pmc-release]
AID - 1741-7007-4-28 [pii]
AID - 10.1186/1741-7007-4-28 [doi]
PST - epublish
SO  - BMC Biol. 2006 Aug 18;4:28. doi: 10.1186/1741-7007-4-28.