PMID- 16919239
OWN - NLM
STAT- MEDLINE
DCOM- 20061019
LR  - 20211203
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 348
IP  - 4
DP  - 2006 Oct 6
TI  - Involvement of phospholipase D1 in collagen type I production of human dermal 
      fibroblasts.
PG  - 1398-402
AB  - In the current study, the involvement of phospholipase D (PLD) in the regulation 
      of collagen type I production was examined using human dermal fibroblasts. 
      Procollagen I production in the cells overexpressing PLD1, but not PLD2, was 
      found to be increased compared with those in the vector control cells. To 
      investigate the role of PLD1, we examined the effect of knockdown of endogenous 
      PLD1 by small interference RNA (siRNA) on collagen production. The reduction of 
      expression levels of PLD1 by siRNA transfection was accompanied by diminution of 
      procollagen biosynthesis and also ribosomal S6 kinase 1 (S6K1) phosphorylation. 
      The activity of mammalian target of rapamycin (mTOR) is essential for 
      phosphorylation of S6K1 and the treatment of dermal fibroblasts with rapamycin, a 
      potent inhibitor of mTOR abolished procollagen I production. These results 
      suggest that PLD1 plays a crucial role in collagen type I production through mTOR 
      signaling in human dermal fibroblast.
FAU - Ohguchi, Kenji
AU  - Ohguchi K
AD  - Gifu International Institute of Biotechnology, 1-1 Naka-Fudogaoka, Kakamigahara, 
      Gifu 504-0838, Japan. kohguchi@giib.or.jp
FAU - Banno, Yoshiko
AU  - Banno Y
FAU - Akao, Yukihiro
AU  - Akao Y
FAU - Nozawa, Yoshinori
AU  - Nozawa Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060810
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Collagen Type I)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 3.1.4.- (phospholipase D2)
RN  - EC 3.1.4.4 (Phospholipase D)
RN  - EC 3.1.4.4 (phospholipase D1)
SB  - IM
MH  - Cells, Cultured
MH  - Collagen Type I/*biosynthesis
MH  - Dermis/cytology
MH  - Fibroblasts/*enzymology/metabolism
MH  - Humans
MH  - Phospholipase D/antagonists & inhibitors/genetics/metabolism/*physiology
MH  - Protein Kinases/metabolism
MH  - RNA Interference
MH  - TOR Serine-Threonine Kinases
EDAT- 2006/08/22 09:00
MHDA- 2006/10/20 09:00
CRDT- 2006/08/22 09:00
PHST- 2006/07/28 00:00 [received]
PHST- 2006/08/02 00:00 [accepted]
PHST- 2006/08/22 09:00 [pubmed]
PHST- 2006/10/20 09:00 [medline]
PHST- 2006/08/22 09:00 [entrez]
AID - S0006-291X(06)01784-0 [pii]
AID - 10.1016/j.bbrc.2006.08.002 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2006 Oct 6;348(4):1398-402. doi: 
      10.1016/j.bbrc.2006.08.002. Epub 2006 Aug 10.