PMID- 1692094
OWN - NLM
STAT- MEDLINE
DCOM- 19900606
LR  - 20190510
IS  - 0027-8874 (Print)
IS  - 0027-8874 (Linking)
VI  - 82
IP  - 10
DP  - 1990 May 16
TI  - Growth inhibition of murine melanoma and human colon carcinoma by recombinant 
      human platelet factor 4.
PG  - 848-53
AB  - Although it is well established that angiogenesis is essential to tumor 
      development, no human protein with high specificity and efficacy for prevention 
      of angiogenesis has been characterized. In a previous study, we demonstrated that 
      recombinant platelet factor 4 (rPF 4) inhibited angiogenesis in the chicken 
      chorioallantoic membrane. In the present study, we have extended that finding to 
      the use of recombinant human platelet factor 4 (rHuPF 4) to inhibit solid tumor 
      growth in the mouse. rHuPF 4 effectively suppressed the growth of the B16-F10 
      murine melanoma in syngeneic C57BL/6J hosts and prevented the growth of primary 
      tumors of both B16-F10 murine melanoma and HCT 116 human colon carcinoma in 
      semisyngeneic CByB6F1/J female athymic nude mice. These two transformed cell 
      lines were completely insensitive to rHuPF 4 in vitro at levels (50 
      micrograms/mL) that extensively inhibit normal endothelial cell proliferation. 
      The migration of human endothelial cells was also inhibited at these 
      concentrations of rHuPF 4, suggesting a second mechanism by which rHuPF 4 may 
      modulate capillary development. The observed antitumor effects of rHuPF 4 might 
      be due to the inhibition of angiogenesis. This finding could have implications 
      for the development of novel therapeutic approaches to angiogenic diseases. 
      Alternative, and possibly concurrent, mechanisms of the rHuPF 4 antitumor effect 
      include lymphokine-activated killer cell activation and the induction of other 
      cytokines.
FAU - Sharpe, R J
AU  - Sharpe RJ
AD  - Repligen Corporation, Cambridge, MA 02139.
FAU - Byers, H R
AU  - Byers HR
FAU - Scott, C F
AU  - Scott CF
FAU - Bauer, S I
AU  - Bauer SI
FAU - Maione, T E
AU  - Maione TE
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Natl Cancer Inst
JT  - Journal of the National Cancer Institute
JID - 7503089
RN  - 0 (Recombinant Proteins)
RN  - 37270-94-3 (Platelet Factor 4)
SB  - IM
MH  - Animals
MH  - Colonic Neoplasms/*pathology/therapy
MH  - Female
MH  - Humans
MH  - Melanoma, Experimental/*pathology/therapy
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neoplasm Transplantation
MH  - *Neovascularization, Pathologic
MH  - Platelet Factor 4/*pharmacology/therapeutic use
MH  - Recombinant Proteins/pharmacology/therapeutic use
MH  - Tumor Cells, Cultured
EDAT- 1990/05/16 00:00
MHDA- 1990/05/16 00:01
CRDT- 1990/05/16 00:00
PHST- 1990/05/16 00:00 [pubmed]
PHST- 1990/05/16 00:01 [medline]
PHST- 1990/05/16 00:00 [entrez]
AID - 10.1093/jnci/82.10.848 [doi]
PST - ppublish
SO  - J Natl Cancer Inst. 1990 May 16;82(10):848-53. doi: 10.1093/jnci/82.10.848.