PMID- 16922713
OWN - NLM
STAT- MEDLINE
DCOM- 20070404
LR  - 20221207
IS  - 0742-3071 (Print)
IS  - 0742-3071 (Linking)
VI  - 23
IP  - 9
DP  - 2006 Sep
TI  - Relationship between common functional polymorphisms of the p22phox gene (-930A > 
      G and +242C > T) and nephropathy as a result of Type 2 diabetes in a Chinese 
      population.
PG  - 1037-41
AB  - OBJECTIVE: Genetic determinants are important in diabetic nephropathy (DN). 
      Oxidative stress has also emerged as an important pathogenic factor in DN and 
      vascular NADH oxidase is a major source of reactive oxygen species (ROS). 
      Previous small studies reported a strong but contradictory association between 
      functional genetic variation of p22(phox), an important subcomponent of NADH 
      oxidase, and DN. We investigated the association between two common functional 
      single nucleotide polymorphisms (SNPs) (-930 A > G and +242 C > T) and DN in a 
      much larger group of Chinese patients with Type 2 diabetes mellitus (T2DM). 
      RESEARCH DESIGN AND METHODS: Case-control study of Chinese subjects with 
      long-standing T2DM (> 10 years). Cases (n = 306) were subjects with a spot 
      urinary albumin : creatinine ratio (ACR) of > 113 mg/mmol or elevated serum 
      creatinine. Control subjects (n = 306) had ACR < 3.3 mg/mmol and normal serum 
      creatinine. Genotyping was carried out by standard PCR and restriction fragment 
      length polymorphism analysis. RESULTS: Gender distribution, age, duration of 
      diabetes and HbA(1c) were similar in cases and control subjects. Distribution of 
      genotypes in the control subjects for both SNPs was consistent with the 
      Hardy-Weinberg equilibrium. Distribution of genotypes did not differ 
      significantly between cases and control subjects for both polymorphisms-+2424C > 
      T: cases CC 84.6%, CT 15.0%, TT 0.4% and control subjects CC 87.6%, CT 11.8%, TT 
      0.6% (P = 0.45); -930 A > G: cases AA 40.5%, AG 41.8%, GG 17.7% and control 
      subjects AA 38.2%, AG 49.0%, GG 12.8% (P = 0.12). Distribution of alleles was 
      also similar-+2424 C > T: cases C 92.2%, T 7.8% and control subjects C 93.5%, T 
      6.5% (P = 0.66); -930 A > G cases A 61.4%, G 38.6% and control subjects A 62.7%, 
      G 37.3% (P = 0.38). We estimated that our study has approximately 80% power to 
      detect a relative risk of 1.65 (for +242 C > T) and 1.35 (for -930 A > G) 
      conferred by the minor allele, respectively. CONCLUSIONS: In contrast with 
      previous small studies, our data suggest that these SNPs do not confer 
      significantly increased susceptibility to DN secondary to T2DM in Chinese 
      subjects.
FAU - Lim, S C
AU  - Lim SC
AD  - Department of Medicine, Alexandra Hospital, Singapore. Su_chi_lim@alexhosp.com.sg
FAU - Goh, S K
AU  - Goh SK
FAU - Lai, Y R
AU  - Lai YR
FAU - Tee, W W
AU  - Tee WW
FAU - Koh, A
AU  - Koh A
FAU - Xu, X H
AU  - Xu XH
FAU - Wu, Y S
AU  - Wu YS
FAU - Yap, E
AU  - Yap E
FAU - Subramaniam, T
AU  - Subramaniam T
FAU - Sum, C F
AU  - Sum CF
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
PL  - England
TA  - Diabet Med
JT  - Diabetic medicine : a journal of the British Diabetic Association
JID - 8500858
RN  - EC 1.6.3.- (NADPH Oxidases)
RN  - EC 1.6.3.1 (CYBA protein, human)
SB  - IM
MH  - Aged
MH  - Asian People/*genetics
MH  - Case-Control Studies
MH  - Diabetes Mellitus, Type 2/ethnology/*genetics
MH  - Diabetic Nephropathies/ethnology/*genetics
MH  - Female
MH  - Gene Frequency
MH  - Genetic Predisposition to Disease
MH  - Genotype
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - NADPH Oxidases/*genetics
MH  - *Polymorphism, Single Nucleotide
MH  - Singapore/epidemiology
EDAT- 2006/08/23 09:00
MHDA- 2007/04/05 09:00
CRDT- 2006/08/23 09:00
PHST- 2006/08/23 09:00 [pubmed]
PHST- 2007/04/05 09:00 [medline]
PHST- 2006/08/23 09:00 [entrez]
AID - DME1916 [pii]
AID - 10.1111/j.1464-5491.2006.01916.x [doi]
PST - ppublish
SO  - Diabet Med. 2006 Sep;23(9):1037-41. doi: 10.1111/j.1464-5491.2006.01916.x.