PMID- 16922869
OWN - NLM
STAT- MEDLINE
DCOM- 20061003
LR  - 20081121
IS  - 1462-5814 (Print)
IS  - 1462-5814 (Linking)
VI  - 8
IP  - 9
DP  - 2006 Sep
TI  - Directed delivery of heat-labile enterotoxin by enterotoxigenic Escherichia coli.
PG  - 1516-27
AB  - Enterotoxigenic Escherichia coli (ETEC), leading causes of diarrhoeal morbidity 
      and mortality in developing countries, are heterogenous pathogens that elaborate 
      heat-labile (LT) and/or heat-stable (ST) enterotoxins which elicit watery, 
      cholera-like diarrhoea. The molecular events permitting efficient delivery of LT 
      remain undefined. Here, we characterize the role of host-pathogen interaction as 
      it relates to the delivery of LT by ETEC. Separation of bacteria from target 
      intestinal epithelial monolayers by semipermeable filters prevented activation of 
      adenylate cyclase suggesting that pathogen-host cell contact is required for 
      efficient toxin delivery. Likewise, a non-motile strain bearing a mutation in the 
      flagellar fliD gene was deficient in delivery of LT relative to the ETEC (H10407) 
      prototype. Although LT secretion via the type II secretion system (T2SS) was 
      responsive to a variety of environmental factors, neither toxin release nor 
      delivery depended on transcriptional activation of genes encoding LT or the T2SS. 
      Fusions of green fluorescent protein to GspM (a component of the T2SS system for 
      LT) and to LT demonstrated that both T2SS and toxin are distributed at one pole 
      of the ETEC bacterium. Optimal LT delivery may occur in a polarized fashion with 
      transfer of preformed toxin upon close interaction with host cells, preventing 
      neutralization of LT.
FAU - Dorsey, F Chuck
AU  - Dorsey FC
AD  - Research Service, Veterans Affairs Medical Center, Memphis, TN, USA.
FAU - Fischer, Julia F
AU  - Fischer JF
FAU - Fleckenstein, James M
AU  - Fleckenstein JM
LA  - eng
GR  - RR-016190/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - India
TA  - Cell Microbiol
JT  - Cellular microbiology
JID - 100883691
RN  - 0 (Enterotoxins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Caco-2 Cells
MH  - Cell Line, Tumor
MH  - Enterotoxins/chemistry/genetics/*metabolism
MH  - Epithelial Cells/metabolism/pathology/ultrastructure
MH  - Escherichia coli/*genetics/metabolism/ultrastructure
MH  - Escherichia coli Proteins/chemistry/genetics/*metabolism
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Hot Temperature
MH  - Humans
MH  - Microscopy, Electron, Scanning
MH  - Microscopy, Fluorescence/methods
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Transfection/instrumentation/methods
EDAT- 2006/08/23 09:00
MHDA- 2006/10/04 09:00
CRDT- 2006/08/23 09:00
PHST- 2006/08/23 09:00 [pubmed]
PHST- 2006/10/04 09:00 [medline]
PHST- 2006/08/23 09:00 [entrez]
AID - CMI736 [pii]
AID - 10.1111/j.1462-5822.2006.00736.x [doi]
PST - ppublish
SO  - Cell Microbiol. 2006 Sep;8(9):1516-27. doi: 10.1111/j.1462-5822.2006.00736.x.