PMID- 16923898
OWN - NLM
STAT- MEDLINE
DCOM- 20061002
LR  - 20181113
IS  - 0021-9193 (Print)
IS  - 1098-5530 (Electronic)
IS  - 0021-9193 (Linking)
VI  - 188
IP  - 17
DP  - 2006 Sep
TI  - Proline betaine uptake in Sinorhizobium meliloti: Characterization of Prb, an 
      opp-like ABC transporter regulated by both proline betaine and salinity stress.
PG  - 6308-17
AB  - Sinorhizobium meliloti uses proline betaine (PB) as an osmoprotectant when 
      osmotically stressed and as an energy source in low-osmolarity environments. To 
      fulfill this dual function, two separate PB transporters, BetS and Hut, that 
      contribute to PB uptake at high and low osmolarity, respectively, have been 
      previously identified. Here, we characterized a novel transport system that 
      mediates the uptake of PB at both high and low osmolarities. Sequence analysis of 
      Tn5-luxAB chromosomal insertions from several PB-inducible mutants has revealed 
      the presence of a four-gene locus encoding the components of an ABC transporter, 
      Prb, which belongs to the oligopeptide permease (Opp) family. Surprisingly, prb 
      mutants were impaired in their ability to transport PB, and oligopeptides were 
      not shown to be competitors for PB uptake. Further analysis of Prb specificity 
      has shown its ability to take up other quaternary ammonium compounds such as 
      choline and, to a lesser extent, glycine betaine. Interestingly, salt stress and 
      PB were found to control prb expression in a positive and synergistic way and to 
      increase Prb transport activity. At low osmolarity, Prb is largely implicated in 
      PB uptake by stationary-phase cells, likely to provide PB as a source of carbon 
      and nitrogen. Furthermore, at high osmolarity, the analysis of prb and betS 
      single and double mutants demonstrated that Prb, together with BetS, is a key 
      system for protection by PB.
FAU - Alloing, Genevieve
AU  - Alloing G
AD  - Unite Interactions Plantes-Microorganismes et Sante Vegetale, UMR6192 
      CNRS-INRA-Universite de Nice Sophia Antipolis, Centre INRA Agrobiotech, 400 Route 
      des Chappes, BP167, 06903 Sophia Antipolis Cedex, France. alloing@unice.fr
FAU - Travers, Isabelle
AU  - Travers I
FAU - Sagot, Brice
AU  - Sagot B
FAU - Le Rudulier, Daniel
AU  - Le Rudulier D
FAU - Dupont, Laurence
AU  - Dupont L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Bacteriol
JT  - Journal of bacteriology
JID - 2985120R
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Oligopeptides)
RN  - 3SCV180C9W (Betaine)
RN  - 9DLQ4CIU6V (Proline)
RN  - N91BDP6H0X (Choline)
RN  - S1L688345C (stachydrine)
SB  - IM
MH  - ATP-Binding Cassette Transporters/genetics/*physiology
MH  - Adaptation, Physiological
MH  - Betaine/metabolism
MH  - Choline/metabolism
MH  - Gene Expression Regulation, Bacterial
MH  - Membrane Transport Proteins/genetics/*physiology
MH  - Oligopeptides
MH  - Osmolar Concentration
MH  - Proline/*analogs & derivatives/metabolism
MH  - Sinorhizobium meliloti/genetics/*metabolism
PMC - PMC1595395
EDAT- 2006/08/23 09:00
MHDA- 2006/10/03 09:00
PMCR- 2007/03/01
CRDT- 2006/08/23 09:00
PHST- 2006/08/23 09:00 [pubmed]
PHST- 2006/10/03 09:00 [medline]
PHST- 2006/08/23 09:00 [entrez]
PHST- 2007/03/01 00:00 [pmc-release]
AID - 188/17/6308 [pii]
AID - 0585-06 [pii]
AID - 10.1128/JB.00585-06 [doi]
PST - ppublish
SO  - J Bacteriol. 2006 Sep;188(17):6308-17. doi: 10.1128/JB.00585-06.