PMID- 16924124
OWN - NLM
STAT- MEDLINE
DCOM- 20070109
LR  - 20181113
IS  - 0022-1554 (Print)
IS  - 1551-5044 (Electronic)
IS  - 0022-1554 (Linking)
VI  - 54
IP  - 12
DP  - 2006 Dec
TI  - Chromosome-specific DNA repeat probes.
PG  - 1363-70
AB  - In research as well as in clinical applications, fluorescence in situ 
      hybridization (FISH) has gained increasing popularity as a highly sensitive 
      technique to study cytogenetic changes. Today, hundreds of commercially available 
      DNA probes serve the basic needs of the biomedical research community. Widespread 
      applications, however, are often limited by the lack of appropriately labeled, 
      specific nucleic acid probes. We describe two approaches for an expeditious 
      preparation of chromosome-specific DNAs and the subsequent probe labeling with 
      reporter molecules of choice. The described techniques allow the preparation of 
      highly specific DNA repeat probes suitable for enumeration of chromosomes in 
      interphase cell nuclei or tissue sections. In addition, there is no need for 
      chromosome enrichment by flow cytometry and sorting or molecular cloning. Our 
      PCR-based method uses either bacterial artificial chromosomes or human genomic 
      DNA as templates with alpha-satellite-specific primers. Here we demonstrate the 
      production of fluorochrome-labeled DNA repeat probes specific for human 
      chromosomes 17 and 18 in just a few days without the need for highly specialized 
      equipment and without the limitation to only a few fluorochrome labels.
FAU - Baumgartner, Adolf
AU  - Baumgartner A
AD  - Department of Obstetrics, Gynecology and Reproductive Sciences, University of 
      California, San Francisco, California, CA 94720, USA.
FAU - Weier, Jingly Fung
AU  - Weier JF
FAU - Weier, Heinz-Ulrich G
AU  - Weier HU
LA  - eng
SI  - GENBANK/AC131274
SI  - GENBANK/AC136363
GR  - HD-041425/HD/NICHD NIH HHS/United States
GR  - HD-045736/HD/NICHD NIH HHS/United States
GR  - K25 HD041425/HD/NICHD NIH HHS/United States
GR  - CA-88258/CA/NCI NIH HHS/United States
GR  - R01 HD045736/HD/NICHD NIH HHS/United States
GR  - CA-80792/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20060821
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (DNA Probes)
SB  - IM
MH  - Chromosomes, Artificial, Bacterial/*genetics
MH  - Chromosomes, Human, Pair 17/*genetics
MH  - Chromosomes, Human, Pair 18/*genetics
MH  - Cloning, Molecular
MH  - *DNA Probes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction/methods
MH  - Repetitive Sequences, Nucleic Acid
MH  - Sensitivity and Specificity
PMC - PMC3958121
EDAT- 2006/08/23 09:00
MHDA- 2007/01/11 09:00
PMCR- 2007/12/01
CRDT- 2006/08/23 09:00
PHST- 2006/08/23 09:00 [pubmed]
PHST- 2007/01/11 09:00 [medline]
PHST- 2006/08/23 09:00 [entrez]
PHST- 2007/12/01 00:00 [pmc-release]
AID - jhc.6A6974.2006 [pii]
AID - 10.1369_jhc.6A6974.2006 [pii]
AID - 10.1369/jhc.6A6974.2006 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 2006 Dec;54(12):1363-70. doi: 10.1369/jhc.6A6974.2006. Epub 
      2006 Aug 21.