PMID- 16925768
OWN - NLM
STAT- MEDLINE
DCOM- 20060921
LR  - 20131121
IS  - 1464-4096 (Print)
IS  - 1464-4096 (Linking)
VI  - 98
IP  - 3
DP  - 2006 Sep
TI  - Oxalate ions and calcium oxalate crystal-induced up-regulation of osteopontin and 
      monocyte chemoattractant protein-1 in renal fibroblasts.
PG  - 656-60
AB  - OBJECTIVE: To examine the responses of renal fibroblasts to high oxalate (Ox) and 
      calcium Ox (CaOx) crystals, as the latter are found in the renal interstitium of 
      patients with primary or enteric hyperoxaluria, and in animals with experimental 
      CaOx nephrolithiasis, and are associated with tubulointerstitial inflammation 
      (TI). TI might begin with the production of chemoattractants by the renal 
      epithelial cells exposed to high Ox and/or CaOx crystals; as Ox levels are also 
      high in the renal interstitium and crystal deposition in nephrolithiasis might 
      start in the interstitium, we hypothesized that renal fibroblasts might also be 
      involved in the development of TI. MATERIALS AND METHODS: We exposed renal 
      fibroblast cells of line NRK 49F in vitro to Ox ions (500 micromol/L) or CaOx 
      monohydrate crystals (67 microg/cm(2)). We assessed the production of osteopontin 
      and monocyte chemoattractant protein-1 (MCP-1), and expression of their mRNA, in 
      the cells. We also determined the cellular malondialdehyde content as a marker of 
      reactive oxygen species (ROS)-induced lipid peroxidation, and Trypan blue 
      staining and the release of lactate dehydrogenase as markers of injury. RESULTS: 
      Similar to renal epithelial cells, renal fibroblasts were stimulated by exposure 
      to Ox and CaOx crystals. They showed signs of injury and ROS-induced lipid 
      peroxidation. The mRNA expression and production of osteopontin and MCP-1 
      increased significantly. CONCLUSIONS: These results indicate that fibroblasts 
      respond to high Ox and CaOx crystals by up-regulating specific pathways producing 
      pro-inflammatory conditions. Migration of monocytes/macrophages to sites of 
      interstitial crystal deposits can lead to localized interstitial inflammation and 
      fibrosis.
FAU - Umekawa, Tohru
AU  - Umekawa T
AD  - Department of Urology, Kinki University, School of Medicine, Osaka, Japan.
FAU - Iguchi, Masanori
AU  - Iguchi M
FAU - Uemura, Hirotsugu
AU  - Uemura H
FAU - Khan, Saeed R
AU  - Khan SR
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - England
TA  - BJU Int
JT  - BJU international
JID - 100886721
RN  - 0 (Chemokine CCL2)
RN  - 0 (Ions)
RN  - 0 (Oxalates)
RN  - 0 (Sialoglycoproteins)
RN  - 0 (Spp1 protein, rat)
RN  - 106441-73-0 (Osteopontin)
RN  - 2612HC57YE (Calcium Oxalate)
SB  - IM
MH  - Animals
MH  - Calcium Oxalate/metabolism
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - Crystallization
MH  - Fibroblasts/metabolism
MH  - Ions
MH  - Kidney/cytology/*metabolism
MH  - Osteopontin
MH  - Oxalates/*metabolism
MH  - Rats
MH  - Sialoglycoproteins/*metabolism
MH  - Up-Regulation
EDAT- 2006/08/24 09:00
MHDA- 2006/09/22 09:00
CRDT- 2006/08/24 09:00
PHST- 2006/08/24 09:00 [pubmed]
PHST- 2006/09/22 09:00 [medline]
PHST- 2006/08/24 09:00 [entrez]
AID - BJU6334 [pii]
AID - 10.1111/j.1464-410X.2006.06334.x [doi]
PST - ppublish
SO  - BJU Int. 2006 Sep;98(3):656-60. doi: 10.1111/j.1464-410X.2006.06334.x.