PMID- 16926163
OWN - NLM
STAT- MEDLINE
DCOM- 20061127
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 281
IP  - 41
DP  - 2006 Oct 13
TI  - Differentiation arrest by hypoxia.
PG  - 30678-83
AB  - The stem cell niche is a unique tissue microenvironment that regulates the 
      self-renewal and differentiation of stem cells. Although several stromal cells 
      and molecular pathways have been identified, the microenvironment of the stem 
      cell niche remains largely unclear. Recent evidence suggests that stem cells are 
      localized in areas with low oxygen. We have hypothesized that hypoxia maintains 
      the undifferentiated phenotype of stem/precursor cells. In this report, we 
      demonstrate that hypoxia reversibly arrests preadipocytes in an undifferentiated 
      state. Consistent with this observation, hypoxia maintains the expression of 
      pref-1, a key stem/precursor cell gene that negatively regulates adipogenic 
      differentiation. We further demonstrate that the hypoxia-inducible factor-1 
      (HIF-1) constitutes an important mechanism for the inhibition of adipogenic 
      differentiation by hypoxia. Our findings suggest that hypoxia in the stem cell 
      niche is critical for the maintenance of the undifferentiated stem or precursor 
      cell phenotype.
FAU - Lin, Qun
AU  - Lin Q
AD  - Department of Therapeutic Radiology, Yale University School of Medicine, New 
      Haven, Connecticut 06510, USA.
FAU - Lee, Yi-Jang
AU  - Lee YJ
FAU - Yun, Zhong
AU  - Yun Z
LA  - eng
PT  - Journal Article
DEP - 20060822
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Hif1a protein, mouse)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/metabolism
MH  - Adipose Tissue/cytology/metabolism
MH  - Animals
MH  - Cell Differentiation
MH  - *Hypoxia
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Oxygen/metabolism
MH  - Phenotype
MH  - Stem Cells/cytology
EDAT- 2006/08/24 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/08/24 09:00
PHST- 2006/08/24 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/08/24 09:00 [entrez]
AID - S0021-9258(19)89282-7 [pii]
AID - 10.1074/jbc.C600120200 [doi]
PST - ppublish
SO  - J Biol Chem. 2006 Oct 13;281(41):30678-83. doi: 10.1074/jbc.C600120200. Epub 2006 
      Aug 22.