PMID- 16928598
OWN - NLM
STAT- MEDLINE
DCOM- 20061026
LR  - 20191210
IS  - 0953-7104 (Print)
IS  - 0953-7104 (Linking)
VI  - 17
IP  - 5
DP  - 2006 Aug
TI  - Endogenously generated reactive oxygen species reduce PMCA activity in platelets 
      from patients with non-insulin-dependent diabetes mellitus.
PG  - 283-8
AB  - Intracellular Ca2+ homeostasis in platelets of patients with 
      non-insulin-dependent diabetes mellitus (NIDDM) has been reported to be altered, 
      leading to an increased adhesiveness and spontaneous aggregation. Among the 
      disturbed Ca2+ mechanism in platelets from NIDDM subjects, a reduced Ca2+ 
      extrusion by the plasma membrane Ca2+-ATPase (PMCA) is especially relevant, 
      maintaining an elevated cytosolic free Ca2+ concentration that results in 
      platelet hypersensitivity. Here we show that treatment of platelets from NIDDM 
      patients with 300 U/mL catalase or 5 mM D-mannitol, which prevent H2O2- and 
      hydroxyl radicals-mediated oxidative stress, respectively, increases Ca2+ 
      extrusion after treatment with thapsigargin (TG) plus ionomycin (Iono). In 
      contrast, 1 mM trolox, a scavenger of ONOO-, did not alter TG + Iono-induced 
      response. Catalase and D-mannitol reversed the enhanced tyrosine phosphorylation 
      of PMCA induced by TG + Iono in NIDDM patients. These findings open up new 
      horizon for the development of therapeutic strategies to palliate cardiovascular 
      disorders in NIDDM.
FAU - Jardin, Isaac
AU  - Jardin I
AD  - Department of Physiology, University of Extremadura, 10071 Caceres, Spain.
FAU - Redondo, Pedro C
AU  - Redondo PC
FAU - Salido, Gines M
AU  - Salido GM
FAU - Pariente, Jose A
AU  - Pariente JA
FAU - Rosado, Juan A
AU  - Rosado JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Platelets
JT  - Platelets
JID - 9208117
RN  - 0 (Antioxidants)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Ionophores)
RN  - 0 (Reactive Oxygen Species)
RN  - 21820-51-9 (Phosphotyrosine)
RN  - 56092-81-0 (Ionomycin)
RN  - 67526-95-8 (Thapsigargin)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Antioxidants/pharmacology
MH  - Blood Platelets/drug effects/*enzymology
MH  - Calcium/*metabolism
MH  - Calcium-Transporting ATPases/blood/*drug effects
MH  - Catalase/metabolism
MH  - Cell Membrane/metabolism
MH  - Diabetes Mellitus, Type 2/*blood/enzymology
MH  - Enzyme Inhibitors/pharmacology
MH  - Humans
MH  - Ionomycin/pharmacology
MH  - Ionophores/pharmacology
MH  - Phosphotyrosine/analysis
MH  - *Platelet Activation/drug effects/physiology
MH  - Reactive Oxygen Species/*metabolism
MH  - Thapsigargin/pharmacology
EDAT- 2006/08/25 09:00
MHDA- 2006/10/27 09:00
CRDT- 2006/08/25 09:00
PHST- 2006/08/25 09:00 [pubmed]
PHST- 2006/10/27 09:00 [medline]
PHST- 2006/08/25 09:00 [entrez]
AID - U733670329825557 [pii]
AID - 10.1080/09537100600745187 [doi]
PST - ppublish
SO  - Platelets. 2006 Aug;17(5):283-8. doi: 10.1080/09537100600745187.