PMID- 16929832
OWN - NLM
STAT- MEDLINE
DCOM- 20110407
LR  - 20140625
VI  - 20
IP  - 11
DP  - 2006 Jun
TI  - [The expression and DNA sequence of recombination activating gene-1 in nasal 
      membrane of allergic rhinitis].
PG  - 505-7, 510
AB  - OBJECTIVE: To study the mechanism of recombination activating gene-1 in 
      pathogenesis of allergic rhinitis and its relation to interleukin-4 (IL-4), 
      interleukin-10 (IL-10) and immunoglobin E (IgE). METHOD: Three groups of allergic 
      rats models were established with ovalbumin (OVA) and intervened with 
      Dexamethasone (DEX). The times of sneeze were recorded, the cutaneous reaction of 
      rats to the passive cutaneous allergen access (PCA) were investigated and the 
      expression and DNA sequence of recombination activating gene-1 were examined and 
      tested with reverse transcription-polymerase chain reaction (RT-PCR) and DNA 
      analysator. The serum levels of IL-4, IL-10 and IgE were examined and tested with 
      enzyme linked immunosorbent assay (ELISA) method in comparison with the controls. 
      RESULT: RAG-1 mRNA expression was not found in normal nasal membrane. Both in AR 
      group and DEX intervention group were seen different expressions; DNA sequence 
      determination didn't find mutation of RG1 genes. The serum level of IL-4[(106.31 
      +/- 12.90) ng/L] and IgE[(38.67 +/- 4.13) ng/L] in AR group were significantly 
      higher than those in normal control [(93.65 +/- 7.78) ng/L, (23.27 +/- 1.36) ng/L 
      respectively] (P < 0.05), but the IL-10 level [(38.15 +/- 4.89) ng/L] in AR group 
      was significantly lower than those in normal control [(48.74 +/- 3.49) ng/L] (P < 
      0.05). After DEX intervention, the serum level of IL-4[(92.67 +/- 16.40) ng/L] 
      and IgE[(24.23 +/- 4.38) ng/L] significantly dropped as compared with AR group (P 
      < 0.05) while IL-10[(46.18 +/- 5.01) ng/L] significantly rose (P < 0.05). 
      CONCLUSION: It proves that the high expression of recombination activating genes 
      is closely related to the pathogenesis of allergic rhinitis, which leads to the 
      secretion of IL-4 and inhibits that of IL-10 and whose sequence is conservative 
      in the nasal mucosa of AR. As glucocorticoid does not affect the expression of 
      RAG-1 genes, it suggests that RAG-1 may be an immunomodulator on a higher level 
      in the pathogenesis of AR.
FAU - Zhang, Ruxin
AU  - Zhang R
AD  - Department of Otolaryngology, Changhai Hospital, the Second Military Medical 
      University, Shanghai, 200433, China. rxzhang@X263.com
FAU - Wu, Geping
AU  - Wu G
FAU - Wen, Wu
AU  - Wen W
FAU - Liu, Guojun
AU  - Liu G
FAU - Yan, Zhiqiang
AU  - Yan Z
FAU - Yu, Shaoqing
AU  - Yu S
FAU - Sun, Wei
AU  - Sun W
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Lin Chuang Er Bi Yan Hou Ke Za Zhi
JT  - Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology
JID - 9426080
RN  - 0 (Homeodomain Proteins)
RN  - 128559-51-3 (RAG-1 protein)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Female
MH  - Gene Expression
MH  - Homeodomain Proteins/*genetics
MH  - Immunoglobulin E/immunology
MH  - Interleukin-10/immunology
MH  - Interleukin-4/immunology
MH  - Male
MH  - Nasal Mucosa/immunology/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rhinitis, Allergic, Perennial/*genetics/immunology/*metabolism
MH  - Sequence Analysis, DNA
EDAT- 2006/08/26 09:00
MHDA- 2011/04/08 06:00
CRDT- 2006/08/26 09:00
PHST- 2006/08/26 09:00 [pubmed]
PHST- 2011/04/08 06:00 [medline]
PHST- 2006/08/26 09:00 [entrez]
PST - ppublish
SO  - Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2006 Jun;20(11):505-7, 510.