PMID- 16929838
OWN - NLM
STAT- MEDLINE
DCOM- 20100707
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 24
IP  - 3
DP  - 2006 May
TI  - [Determination of selenomethionine in selenium-enriched yeast by gas 
      chromatography-mass spectrometry].
PG  - 235-8
AB  - A method has been developed for the determination of selenomethionine in 
      selenium-enriched yeast by gas chromatography-mass spectrometry (GC-MS). Three 
      extraction methods were compared for extraction efficiency of selenomethionine 
      from the samples. Selenomethionine in the samples was extracted for 24 h with 
      proteinase in Tris buffer. The selenomethionine was derivatized with butanol and 
      trifluoroacetic acid (TFA). The derivatization was accomplished in two steps, 
      starting with the esterification of the carboxyl group of the selenoamino acid 
      using butanol, followed by the acylation of the amino group with trifluoroacetic 
      acid anhydride. The selected ion for monitoring selenomethionine was at m/z 349. 
      The instrument operating conditions were optimized. The samples were analyzed by 
      GC-MS with external standard method. Standard GC-MS chromatograms and mass 
      spectra for selenomethionine were also obtained. The method was proved to be 
      accurate and reliable. The recoveries of 98.5%-103.7% with relative standard 
      deviations (RSDs) of 0.9%-2.4% (n = 6) and the correlation coefficient of 0.9978 
      were obtained. The detection limit of selenomethionine for the method was 0.5 
      mg/L (S/N = 3) and the selenomethionine contents of real sample was given.
FAU - Gao, Jianzhong
AU  - Gao J
AD  - College of Veterinary Medicine, Nanjing Agricultural University, China. 
      gsn08@yahoo.com.cn
FAU - Huang, Kehe
AU  - Huang K
FAU - Qin, Shunyi
AU  - Qin S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Nanotubes, Carbon)
RN  - 964MRK2PEL (Selenomethionine)
SB  - IM
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Hydrogen-Ion Concentration
MH  - Limit of Detection
MH  - Nanotubes, Carbon/chemistry
MH  - Selenomethionine/*analysis
MH  - Yeast, Dried/*analysis
EDAT- 2006/08/26 09:00
MHDA- 2010/07/08 06:00
CRDT- 2006/08/26 09:00
PHST- 2006/08/26 09:00 [pubmed]
PHST- 2010/07/08 06:00 [medline]
PHST- 2006/08/26 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2006 May;24(3):235-8.