PMID- 16929841
OWN - NLM
STAT- MEDLINE
DCOM- 20100707
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 24
IP  - 3
DP  - 2006 May
TI  - [Simultaneous determination of four compounds in Sanjing Shuanghuanglian Oral 
      Liquid by high performance liquid chromatography-diode array 
      detection-electrochemical detection].
PG  - 247-50
AB  - Chlorogenic acid, caffeic acid, baicalin and luteolin in Sanjing Shuanghuanglian 
      Oral Liquid were simultaneously detected and identified using a high performance 
      liquid chromatography coupled with diode array detection and electrochemical 
      detection (HPLC-DAD-ECD). The separation was performed on a Zorbax SB-C18 column 
      (150 mm x 4.6 mm i. d., 5.0 microm). The mobile phase consisted of (A) methanol 
      and (B) methanol-water-acetic acid (50: 50: 1, v/v/v) using a linear gradient 
      elution of 2%A-3%A at 0-3 min, 3%A-25%A at 3-15 min, 25%A-80%A at 15-20 min. The 
      flow rate was 0.8 mL/min. The DAD detection was used at 275 nm. The ECD detection 
      was done at 0.7 V. The column thermostat set at 30 degrees C. The limits of 
      detection of the 4 compounds were 1 mg/L for chlorogenic acid, 0.2 mg/L for 
      caffeic acid, 9 mg/L for baicalin, 7 mg/L for luteolin. The average recoveries 
      were between 96.6%-99.6% with relative standard deviations (RSDs) of 2.5%-4.1%. 
      The method is simple, rapid, reproducible and accurate. It can be used for the 
      routine analysis of the four compounds in Shuanghuanglian Oral Liquid.
FAU - Liu, Lin
AU  - Liu L
AD  - Institute of Analytical Science, Shaanxi Provincial Key Laboratory of 
      Electroanalytical Chemistry, Northwest University, Xi'an, China.
FAU - Suo, Zhirong
AU  - Suo Z
FAU - Zheng, Jianbin
AU  - Zheng J
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Caffeic Acids)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Flavonoids)
RN  - 0 (Plant Extracts)
RN  - 0 (Propionates)
RN  - 318ADP12RI (Chlorogenic Acid)
RN  - 347Q89U4M5 (baicalin)
RN  - KUX1ZNC9J2 (Luteolin)
RN  - U2S3A33KVM (caffeic acid)
SB  - IM
MH  - Caffeic Acids/*analysis
MH  - Chlorogenic Acid/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Drugs, Chinese Herbal/*chemistry
MH  - Flavonoids/*analysis
MH  - Luteolin/*analysis
MH  - Medicine, Chinese Traditional
MH  - Plant Extracts/chemistry
MH  - Propionates/analysis
MH  - Spectrophotometry, Ultraviolet/methods
EDAT- 2006/08/26 09:00
MHDA- 2010/07/08 06:00
CRDT- 2006/08/26 09:00
PHST- 2006/08/26 09:00 [pubmed]
PHST- 2010/07/08 06:00 [medline]
PHST- 2006/08/26 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2006 May;24(3):247-50.