PMID- 16930328
OWN - NLM
STAT- MEDLINE
DCOM- 20061103
LR  - 20061115
IS  - 1320-5463 (Print)
IS  - 1320-5463 (Linking)
VI  - 56
IP  - 9
DP  - 2006 Sep
TI  - Repeated fluorescence in situ hybridization by a microwave-enhanced protocol.
PG  - 490-3
AB  - A novel re-hybridization protocol for pathology archive sections that uses 
      microwave-assisted fluorescence in situ hybridization (FISH) is described. 
      Stripping the probe from the pathology archive sections with HCl and 
      re-hybridizing with the next probe by intermittent microwave irradiation 
      generated clear signals without background noise. Repeated stripping and 
      hybridization with numerous bacterial artificial chromosome (BAC)-derived probes 
      would identify the profile of genome-wide changes in small lesions on sections.
FAU - Kitayama, Yasuhiko
AU  - Kitayama Y
AD  - First Department of Pathology, Hamamatsu University School of Medicine, 
      Hamamatsu, Japan.
FAU - Igarashi, Hisaki
AU  - Igarashi H
FAU - Kozu, Taeko
AU  - Kozu T
FAU - Nagura, Kiyoko
AU  - Nagura K
FAU - Ohashi, Yohei
AU  - Ohashi Y
FAU - Sugimura, Haruhiko
AU  - Sugimura H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Pathol Int
JT  - Pathology international
JID - 9431380
SB  - IM
MH  - *Chromosome Aberrations
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Microwaves
MH  - Paraffin Embedding
EDAT- 2006/08/26 09:00
MHDA- 2006/11/04 09:00
CRDT- 2006/08/26 09:00
PHST- 2006/08/26 09:00 [pubmed]
PHST- 2006/11/04 09:00 [medline]
PHST- 2006/08/26 09:00 [entrez]
AID - PIN1996 [pii]
AID - 10.1111/j.1440-1827.2006.01996.x [doi]
PST - ppublish
SO  - Pathol Int. 2006 Sep;56(9):490-3. doi: 10.1111/j.1440-1827.2006.01996.x.