PMID- 16931866
OWN - NLM
STAT- MEDLINE
DCOM- 20070123
LR  - 20190508
IS  - 0022-0345 (Print)
IS  - 1544-0591 (Electronic)
IS  - 0022-0345 (Linking)
VI  - 85
IP  - 9
DP  - 2006 Sep
TI  - S100A8 triggers oxidation-sensitive repulsion of neutrophils.
PG  - 829-33
AB  - The inflammatory response to tissue injury is a multi-faceted process. During 
      this process, neutrophils migrate in the extravascular spaces, directed to the 
      site of injury by chemical gradients generated by chemotactic molecules. S100A8, 
      a protein associated with a wide variety of inflammatory conditions, is heavily 
      over-expressed in association with inflammation. We hypothesized that human 
      S100A8 possesses neutrophil-repelling properties that result in an 
      anti-inflammatory effect in vivo. The chemotactic activity of S100A8 on 
      neutrophils was tested in Transwell chemotaxis assays. Analysis of the data 
      indicates that S100A8 causes a repulsion of peripheral neutrophils, an activity 
      that S100A8 loses upon its oxidation. Using a mutant of S100A8 resistant to 
      oxidation and consistent with the in vitro findings, we demonstrated that S100A8 
      causes a strong anti-inflammatory effect in the rat air-pouch model of 
      inflammation in vivo. These data highlight a naturally occurring novel 
      anti-inflammatory pathway and provide potential molecular targets for the 
      development of novel anti-inflammatory therapeutics. Abbrevations: ethylene 
      diamine tetraacetic acid (EDTA); limulus amoebocyte lysate assay (LAL); pertussis 
      toxin (PTX); forward scatter (FSC); Interleukin-8 (IL-8); formyl-Met-Leu-Phe 
      (fMLP); monocyte chemotactic protein 1 (MCP1).
FAU - Sroussi, H Y
AU  - Sroussi HY
AD  - Department of Oral Medicine and Diagnostic Sciences, University of Illinois at 
      Chicago, College of Dentistry (M/C 838), 801 S. Paulina St., Room 556, Chicago, 
      IL 60612-7213, USA. sroussih@uic.edu
FAU - Berline, J
AU  - Berline J
FAU - Dazin, P
AU  - Dazin P
FAU - Green, P
AU  - Green P
FAU - Palefsky, J M
AU  - Palefsky JM
LA  - eng
GR  - P01 DE 07946/DE/NIDCR NIH HHS/United States
GR  - P01 DE007946/DE/NIDCR NIH HHS/United States
GR  - T32 DE07204/DE/NIDCR NIH HHS/United States
GR  - K16 DE 00386/DE/NIDCR NIH HHS/United States
GR  - K16 DE000386-04/DE/NIDCR NIH HHS/United States
GR  - T32 DE007204/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Dent Res
JT  - Journal of dental research
JID - 0354343
RN  - 0 (Calgranulin A)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Recombinant Proteins)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - K848JZ4886 (Cysteine)
RN  - OF5P57N2ZX (Alanine)
SB  - IM
MH  - Alanine/metabolism
MH  - Animals
MH  - Calgranulin A/*physiology
MH  - Cells, Cultured
MH  - Chemotaxis, Leukocyte/drug effects/*physiology
MH  - Cysteine/metabolism
MH  - Flow Cytometry
MH  - Humans
MH  - Inflammation/*metabolism
MH  - Inflammation Mediators/*antagonists & inhibitors
MH  - Lipopolysaccharides
MH  - N-Formylmethionine Leucyl-Phenylalanine/antagonists & inhibitors
MH  - Neutrophils/drug effects/*physiology
MH  - Oxidation-Reduction
MH  - Rats
MH  - Recombinant Proteins/pharmacology
PMC - PMC2248158
MID - NIHMS38060
EDAT- 2006/08/26 09:00
MHDA- 2007/01/24 09:00
PMCR- 2008/02/19
CRDT- 2006/08/26 09:00
PHST- 2006/08/26 09:00 [pubmed]
PHST- 2007/01/24 09:00 [medline]
PHST- 2006/08/26 09:00 [entrez]
PHST- 2008/02/19 00:00 [pmc-release]
AID - 85/9/829 [pii]
AID - 10.1177/154405910608500910 [doi]
PST - ppublish
SO  - J Dent Res. 2006 Sep;85(9):829-33. doi: 10.1177/154405910608500910.