PMID- 16931898
OWN - NLM
STAT- MEDLINE
DCOM- 20070104
LR  - 20161124
IS  - 1660-5527 (Print)
IS  - 1660-5527 (Linking)
VI  - 19
IP  - 6
DP  - 2006
TI  - Effect of 5alpha-dihydrotestosterone and testosterone on apoptosis in human 
      dermal papilla cells.
PG  - 311-21
AB  - Pathogenetic mechanisms in androgenetic alopecia are not yet fully understood; 
      however, it is commonly accepted that androgens like testosterone (T) and 
      5alpha-dihydrotestosterone (5alpha-DHT) inhibit hair follicle activity with early 
      induction of the catagen. Thus, we investigated the influence of T and 5alpha-DHT 
      on proliferation, cell death and bcl-2/bax expression in cultured dermal papilla 
      cells (DPC) from nonbalding scalp regions of healthy volunteers. T and 5alpha-DHT 
      induced apoptosis in DPC in a dose-dependent and time-related manner; in addition 
      a necrotic effect due to T at 10(-5) M was found. Interestingly, bcl-2 protein 
      expression was decreased in T- and 5alpha-DHT-treated cells, leading to an 
      increase in the bax/bcl-2 ratio. In addition, T and 5alpha-DHT induced 
      proteolytic cleavage of caspase 8 and inhibited proliferation of DPC at 10(-5) M. 
      High concentrations of T and 5alpha-DHT were needed to induce apoptotic effects 
      in DPC. These data suggest that DPC from nonbalding scalp regions do have the 
      capacity to undergo apoptosis, but need a high androgen stimulus. The present 
      study provides an interesting new pathogenetic approach in androgenetic alopecia.
FAU - Winiarska, A
AU  - Winiarska A
AD  - Department of Dermatology and Allergy, Charite-Universitatsmedizin Berlin, 
      Berlin, Germany.
FAU - Mandt, N
AU  - Mandt N
FAU - Kamp, H
AU  - Kamp H
FAU - Hossini, A
AU  - Hossini A
FAU - Seltmann, H
AU  - Seltmann H
FAU - Zouboulis, C C
AU  - Zouboulis CC
FAU - Blume-Peytavi, U
AU  - Blume-Peytavi U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060823
PL  - Switzerland
TA  - Skin Pharmacol Physiol
JT  - Skin pharmacology and physiology
JID - 101188418
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 08J2K08A3Y (Dihydrotestosterone)
RN  - 3XMK78S47O (Testosterone)
RN  - 63231-63-0 (RNA)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspase 8)
RN  - EUY85H477I (thiazolyl blue)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Blotting, Western
MH  - Caspase 3/metabolism
MH  - Caspase 8/metabolism
MH  - Cell Survival/drug effects
MH  - Dihydrotestosterone/*pharmacology
MH  - Genes, bcl-2/physiology
MH  - Humans
MH  - In Vitro Techniques
MH  - Necrosis
MH  - RNA/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Skin/cytology/*drug effects
MH  - Testosterone/*pharmacology
MH  - Tetrazolium Salts
MH  - Thiazoles
MH  - bcl-2-Associated X Protein/physiology
EDAT- 2006/08/26 09:00
MHDA- 2007/01/05 09:00
CRDT- 2006/08/26 09:00
PHST- 2005/09/01 00:00 [received]
PHST- 2006/01/20 00:00 [accepted]
PHST- 2006/08/26 09:00 [pubmed]
PHST- 2007/01/05 09:00 [medline]
PHST- 2006/08/26 09:00 [entrez]
AID - 95251 [pii]
AID - 10.1159/000095251 [doi]
PST - ppublish
SO  - Skin Pharmacol Physiol. 2006;19(6):311-21. doi: 10.1159/000095251. Epub 2006 Aug 
      23.