PMID- 16932066
OWN - NLM
STAT- MEDLINE
DCOM- 20061016
LR  - 20141120
IS  - 1052-9551 (Print)
IS  - 1052-9551 (Linking)
VI  - 15
IP  - 3
DP  - 2006 Sep
TI  - Her-2/neu in barrett esophagus: a comparative study between histology, 
      immunohistochemistry, and fluorescence in situ hybridization.
PG  - 125-30
AB  - Her-2/neu is a protooncogene frequently overexpressed in breast cancer, recently 
      found to be also overexpressed in carcinoma arising on Barrett esophagus (BE). 
      Immunohistochemistry and fluorescence in situ hybridization (FISH) are 
      conventionally used for Her-2 testing in carcinomas, but a single assay is not 
      yet accepted as a "gold standard" in BE. To evaluate the correlation between 
      histopathology variables and gene expression/amplification in the sequence BE-low 
      grade dysplasia-high grade dysplasia-adenocarcinoma, fifty esophageal specimens 
      from patients with a diagnosis of BE (21 BE, 4 low-grade dysplasia, 12 high-grade 
      dysplasia, and 13 adenocarcinomas) were evaluated. Histopathologic evaluation was 
      carried out using hematoxylin and eosin staining. Paraffin-embedded tissues were 
      investigated for Her-2 by immunohistochemistry (HercepTest) and FISH. HercepTest 
      was scored 0, 1+, 2+, and 3+ depending on the percentage (cut off 10%) of 
      membrane staining, whereas gene assessment evaluated by FISH was based on the 
      ratio between Her-2/neu and the 17 chromosome copy number. There was a positive 
      correlation between gene amplification and protein overexpression. No case with 
      HercepTest scoring 0 or 1+ displayed gene amplification, but this was present in 
      20% of cases scoring 2+ and in all cases scoring 3+. Her-2/neu amplification or 
      overexpression was never observed in BE. Gene amplification and overexpression 
      was observed in more than 50% of dysplasias and adenocarcinomas. Her-2/neu 
      amplification/overexpression might be considered as a marker of progression from 
      BE to dysplasia. FISH may represent a useful diagnostic tool to integrate the 
      result of HercepTest for selecting patients for more targeted therapeutic 
      approaches.
FAU - Rossi, Elisa
AU  - Rossi E
AD  - Second Department of Pathology, Spedali Civili, University of Brescia, Brescia, 
      Italy.
FAU - Villanacci, Vincenzo
AU  - Villanacci V
FAU - Bassotti, Gabrio
AU  - Bassotti G
FAU - Casa, Domenico Della
AU  - Casa DD
FAU - Missale, Guido
AU  - Missale G
FAU - Minelli, Luigi
AU  - Minelli L
FAU - Cestari, Renzo
AU  - Cestari R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Diagn Mol Pathol
JT  - Diagnostic molecular pathology : the American journal of surgical pathology, part 
      B
JID - 9204924
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Aged
MH  - Barrett Esophagus/*diagnosis/pathology
MH  - Female
MH  - Gene Amplification
MH  - Gene Expression
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - RNA, Messenger/analysis/metabolism
MH  - Receptor, ErbB-2/*analysis/*genetics
EDAT- 2006/08/26 09:00
MHDA- 2006/10/17 09:00
CRDT- 2006/08/26 09:00
PHST- 2006/08/26 09:00 [pubmed]
PHST- 2006/10/17 09:00 [medline]
PHST- 2006/08/26 09:00 [entrez]
AID - 00019606-200609000-00001 [pii]
AID - 10.1097/01.pdm.0000213455.22527.f7 [doi]
PST - ppublish
SO  - Diagn Mol Pathol. 2006 Sep;15(3):125-30. doi: 10.1097/01.pdm.0000213455.22527.f7.