PMID- 1693267
OWN - NLM
STAT- MEDLINE
DCOM- 19900703
LR  - 20131121
IS  - 0003-9985 (Print)
IS  - 0003-9985 (Linking)
VI  - 114
IP  - 6
DP  - 1990 Jun
TI  - Acridine orange--RNA histofluorescence of sarcomas and small round cell tumors of 
      childhood.
PG  - 585-8
AB  - Forty-nine pediatric malignant neoplasms were stained with acridine orange (AO) 
      fluorochrome to qualitatively evaluate cytoplasmic RNA content. The application 
      of AO as a supplementary stain in surgical pathologic diagnosis is based on the 
      premise that specific neoplastic cell types characteristically and consistently 
      contain few or many cytoplasmic ribosomes. Primitive tumors such as Ewing's 
      sarcoma and primitive neuroectodermal tumors showed negative or low-intensity 
      AO-RNA cytoplasmic staining. Differentiated sarcomas such as rhabdomyosarcomas 
      and lymphomas exhibited moderate to strong AO-RNA cytoplasmic fluorescence. 
      Acridine orange--RNA staining provides an easy, convenient, and inexpensive 
      adjunct in the histopathologic differential diagnosis of sarcomas. It is 
      particularly useful for distinguishing Ewing's sarcomas from other small round 
      cell sarcomas of childhood.
FAU - Pinto, A
AU  - Pinto A
AD  - Department of Pathology, University of Calgary Faculty of Medicine, Canada.
FAU - Sarnat, H B
AU  - Sarnat HB
FAU - Vogler, C
AU  - Vogler C
FAU - Trevenen, C L
AU  - Trevenen CL
FAU - Grant, L H
AU  - Grant LH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Arch Pathol Lab Med
JT  - Archives of pathology & laboratory medicine
JID - 7607091
RN  - 0 (RNA, Neoplasm)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Adolescent
MH  - Child
MH  - Child, Preschool
MH  - Cytoplasm/analysis/ultrastructure
MH  - Female
MH  - Humans
MH  - Male
MH  - Microscopy, Electron
MH  - Microscopy, Fluorescence
MH  - Osteosarcoma/metabolism/pathology/ultrastructure
MH  - RNA, Neoplasm/analysis
MH  - Rhabdomyosarcoma/metabolism/pathology/ultrastructure
MH  - Sarcoma/metabolism/*pathology/ultrastructure
MH  - Sarcoma, Ewing/metabolism/pathology/ultrastructure
MH  - Staining and Labeling
EDAT- 1990/06/01 00:00
MHDA- 1990/06/01 00:01
CRDT- 1990/06/01 00:00
PHST- 1990/06/01 00:00 [pubmed]
PHST- 1990/06/01 00:01 [medline]
PHST- 1990/06/01 00:00 [entrez]
PST - ppublish
SO  - Arch Pathol Lab Med. 1990 Jun;114(6):585-8.