PMID- 16933038
OWN - NLM
STAT- MEDLINE
DCOM- 20061222
LR  - 20060825
IS  - 1389-9333 (Print)
IS  - 1389-9333 (Linking)
VI  - 7
IP  - 3
DP  - 2006
TI  - Allograft semilunar cardiac valves processing and cryopreservation - morphology 
      in scanning electron microscope.
PG  - 167-73
AB  - OBJECTIVE: The most important factors of long term clinical performance of 
      biological heart valve prostheses are methods of processing and cryopreservation. 
      That is why we decided to evaluate the impact of current Allograft Heart Valves 
      (AHV) Bank protocol on valve tissue morphology. Scanning electron microscope 
      (SEM) is a valuable tool for investigation of biological surfaces. In case of 
      cardiac valves it is especially suitable for detection of fine changes in 
      endothelial covering and underlying layers. MATERIAL AND METHODS: "Fresh" aortic 
      and pulmonary AHV samples, harvested from "heart-beating" cadaveric donors, were 
      compared with (1) tissue from AHV obtained from non heart-beating donors, (2) 
      samples stored in 4 degrees C saline for 24 h, (3) antibiotic treated tissue for 
      24 h at 37 degrees C and finally (4) cryopreserved valves, stored in liquid 
      nitrogen (-196 degrees C) for 6-38 months. All samples were dissected, dried with 
      hexamethyldisilazane (HMDS), gold coated, studied and photographed by SEM (Tesla 
      BS 301). RESULTS: Our alternative method of drying samples by the HMDS method 
      proved to be suitable for thin membranes of human semilunar valves. We were able 
      to detect early changes in the endothelium after harvesting, and denudation of 
      the endothelial covering during preservation with and without freezing. 
      CONCLUSION: SEM (using HMDS drying) along with other methods may be helpful for 
      the morphological control of processing, cryopreservation and liquid nitrogen 
      storage of AHV. According to the current findings we have to avoid washing of AHV 
      in saline after harvesting.
FAU - Krs, O
AU  - Krs O
AD  - Department of Anatomy, Charles University in Prague, Faculty of Medicine, Hradec 
      Kralove, Czech Republic.
FAU - Burkert, J
AU  - Burkert J
FAU - Slizova, D
AU  - Slizova D
FAU - Kobylka, P
AU  - Kobylka P
FAU - Spatenka, J
AU  - Spatenka J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Cell Tissue Bank
JT  - Cell and tissue banking
JID - 100965121
SB  - IM
MH  - *Cryopreservation
MH  - Heart Valves/anatomy & histology/*transplantation
MH  - Humans
MH  - Microscopy, Electron, Scanning
MH  - Transplantation, Homologous
EDAT- 2006/08/26 09:00
MHDA- 2006/12/23 09:00
CRDT- 2006/08/26 09:00
PHST- 2004/03/22 00:00 [received]
PHST- 2004/08/05 00:00 [accepted]
PHST- 2006/08/26 09:00 [pubmed]
PHST- 2006/12/23 09:00 [medline]
PHST- 2006/08/26 09:00 [entrez]
AID - 10.1007/s10561-004-1889-y [doi]
PST - ppublish
SO  - Cell Tissue Bank. 2006;7(3):167-73. doi: 10.1007/s10561-004-1889-y.