PMID- 16934355
OWN - NLM
STAT- MEDLINE
DCOM- 20061120
LR  - 20060918
IS  - 0168-1605 (Print)
IS  - 0168-1605 (Linking)
VI  - 111
IP  - 3
DP  - 2006 Oct 1
TI  - Rapid assessment of the physiological status of Streptococcus macedonicus by flow 
      cytometry and fluorescence probes.
PG  - 197-205
AB  - Flow cytometry in combination with fluorescence probes was applied to rapidly 
      assess the physiological status of Streptococcus macedonicus ACA-DC 198, a newly 
      described member of the lactic acid bacteria group with technologically important 
      features (e.g. lantibiotic production). A sonication procedure was developed for 
      disaggregating typical streptococci chains in order to optimize cell preparations 
      for single cell analysis. Single stained live and dead populations of S. 
      macedonicus cells were clearly resolved based on membrane potential by bis-oxonol 
      [DiBAC(4)(3)], membrane integrity by Propidium Iodide (PI) and enzymatic activity 
      as well as membrane integrity by Carboxyfluorescein Diacetate (cFDA). Further, 
      estimation of both live and dead cells by a cFDA/PI two-colour flow cytometric 
      assay showed excellent correlation with the dead cells in the samples 
      (dead(FCM)=0.9945 dead(S)-0.806, R(2)=0.9986 and live(FCM)=-0.978 dead(S)+98.895, 
      R(2)=0.9992). Finally, the assay was applied to study the physiology of S. 
      macedonicus after acid stress. Interestingly, in situ assessment of the 
      physiological status of stressed S. macedonicus cells by flow cytometry and 
      single cell sorting revealed the coexistence of three distinct subpopulations 
      according to their fluorescence labelling behaviour and culturability, 
      representing intact/culturable, permeabilized/dead and potentially injured cells 
      with the latter exhibiting both metabolic activity and membrane permeabilization 
      as well as decreased culturability.
FAU - Papadimitriou, Konstantinos
AU  - Papadimitriou K
AD  - Laboratory of Dairy Research, Department of Food Science and Technology, 
      Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece.
FAU - Pratsinis, Harris
AU  - Pratsinis H
FAU - Nebe-von-Caron, Gerhard
AU  - Nebe-von-Caron G
FAU - Kletsas, Dimitris
AU  - Kletsas D
FAU - Tsakalidou, Effie
AU  - Tsakalidou E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060824
PL  - Netherlands
TA  - Int J Food Microbiol
JT  - International journal of food microbiology
JID - 8412849
RN  - 0 (Coloring Agents)
RN  - 0 (Fluoresceins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Thiobarbiturates)
RN  - 0 (carboxyfluoresceindiacetate)
RN  - 36015-30-2 (Propidium)
RN  - 47623-98-3 (bis(1,3-diethylthiobarbiturate)trimethineoxonol)
SB  - IM
MH  - Cell Membrane Permeability/drug effects/*physiology
MH  - Cell Survival
MH  - Coloring Agents/metabolism
MH  - Flow Cytometry/*methods
MH  - Fluoresceins/metabolism
MH  - Fluorescent Dyes/*metabolism
MH  - Membrane Potentials/drug effects/*physiology
MH  - Propidium/metabolism
MH  - *Streptococcus/growth & development/physiology
MH  - Thiobarbiturates/metabolism
MH  - Time Factors
EDAT- 2006/08/29 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/08/29 09:00
PHST- 2005/08/03 00:00 [received]
PHST- 2006/01/11 00:00 [revised]
PHST- 2006/04/21 00:00 [accepted]
PHST- 2006/08/29 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/08/29 09:00 [entrez]
AID - S0168-1605(06)00254-6 [pii]
AID - 10.1016/j.ijfoodmicro.2006.04.042 [doi]
PST - ppublish
SO  - Int J Food Microbiol. 2006 Oct 1;111(3):197-205. doi: 
      10.1016/j.ijfoodmicro.2006.04.042. Epub 2006 Aug 24.