PMID- 16934628
OWN - NLM
STAT- MEDLINE
DCOM- 20061222
LR  - 20060828
IS  - 0099-2399 (Print)
IS  - 0099-2399 (Linking)
VI  - 32
IP  - 9
DP  - 2006 Sep
TI  - The effects of tumor necrosis factor-alpha, interleukin-1beta, interleukin-6, and 
      transforming growth factor-beta1 on pulp fibroblast mediated collagen 
      degradation.
PG  - 853-61
AB  - Dental pulp destruction is believed to be regulated, in part, by the matrix 
      metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs). Cytokines are 
      believed to be important in the pathogenesis of pulpitis. This study examined the 
      effects that TNF-alpha, IL-1beta, IL-6, and TGF-beta1 have on the collagen 
      degradation mediated by pulp fibroblasts utilizing a cell-mediated collagen 
      degradation assay. Reverse transcriptase-polymerase chain reaction, Western blot 
      analyses, and zymography were utilized to examine multiple MMPs and TIMPs. The 
      collagen degradation mediated by these cells was stimulated by these cytokines. 
      TNF-alpha, IL-1beta, and IL-6 increased the mRNA and/or protein expression of 
      MMP-1, MMP-2, and MMP-3. TGF-beta1 decreased MMP-1 mRNA expression, while only 
      slightly affecting the MMP-2 and MMP-3 mRNA and/or protein. These cytokines did 
      not affect the expression of TIMP-1 or TIMP-2. These results suggest that these 
      cytokines affect pulp destruction, in part, by differentially regulating the MMPs 
      and TIMPs.
FAU - Wisithphrom, Kessiri
AU  - Wisithphrom K
AD  - Department of Oral Biology, Indiana University School of Dentistry, Indianapolis, 
      IN 46202, USA.
FAU - Windsor, L Jack
AU  - Windsor LJ
LA  - eng
PT  - Journal Article
DEP - 20060703
PL  - United States
TA  - J Endod
JT  - Journal of endodontics
JID - 7511484
RN  - 0 (Cytokines)
RN  - 0 (Fibrillar Collagens)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
MH  - Adult
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Cytokines/*physiology
MH  - Dental Pulp/cytology/*enzymology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Enzyme Activation
MH  - Fibrillar Collagens/*metabolism
MH  - Fibroblasts/enzymology
MH  - Gene Expression Regulation, Enzymologic
MH  - Humans
MH  - Interleukin-1/physiology
MH  - Interleukin-6/physiology
MH  - Matrix Metalloproteinases/*biosynthesis
MH  - Pulpitis/*metabolism
MH  - RNA, Messenger/analysis
MH  - Tissue Inhibitor of Metalloproteinases/metabolism
MH  - Transforming Growth Factor beta1/physiology
MH  - Tumor Necrosis Factor-alpha/physiology
EDAT- 2006/08/29 09:00
MHDA- 2006/12/23 09:00
CRDT- 2006/08/29 09:00
PHST- 2006/02/23 00:00 [received]
PHST- 2006/03/16 00:00 [revised]
PHST- 2006/03/17 00:00 [accepted]
PHST- 2006/08/29 09:00 [pubmed]
PHST- 2006/12/23 09:00 [medline]
PHST- 2006/08/29 09:00 [entrez]
AID - S0099-2399(06)00285-8 [pii]
AID - 10.1016/j.joen.2006.03.017 [doi]
PST - ppublish
SO  - J Endod. 2006 Sep;32(9):853-61. doi: 10.1016/j.joen.2006.03.017. Epub 2006 Jul 3.