PMID- 16935246
OWN - NLM
STAT- MEDLINE
DCOM- 20061006
LR  - 20161124
IS  - 1389-1723 (Print)
IS  - 1347-4421 (Linking)
VI  - 101
IP  - 6
DP  - 2006 Jun
TI  - Open L-lactic acid fermentation of food refuse using thermophilic Bacillus 
      coagulans and fluorescence in situ hybridization analysis of microflora.
PG  - 457-63
AB  - In the production of commercially useful poly-L-lactic acid plastic from biomass 
      wastes, a feasible fermentation process to produce optically active L-lactic acid 
      would be required. Here, model kitchen refuse (MKR) was inoculated with Bacillus 
      coagulans NBRC12583 under nonsterilized openculture conditions. At temperatures 
      below 45 degrees C, a racemic mixture of D- and L-lactic acids was accumulated, 
      whereas only L-lactic acid was selectively accumulated by incubation at 50-65 
      degrees C. At 45 degrees C, the results of fermentation could not be consistently 
      reproduced. To analyze microflora in this type of mixed culture system, 
      whole-cell fluorescence in situ hybridization (FISH) using 16S rRNA-targeted 
      oligonucleotide probes for B. coagulans, Bcoa191, and LAC722(L), a group-specific 
      probe for a wide range of mesophilic lactic acid bacteria was applied. The 
      dominancy of mesophilic lactic acid bacteria at lower temperatures, and that of 
      B. coagulans at higher temperatures were confirmed. By using a saccharified 
      liquid of collected kitchen refuse, 86 g/l of L-lactic acid was accumulated under 
      nonsterile conditions by a 5-d incubation at 55 degrees C, pH 6.5, with 53% 
      carbon yield and 97% optical purity. To conclude, high temperature open lactic 
      acid fermentation is a simple and promising method for producing high-grade 
      L-lactic acid from biomass waste, and FISH analysis of such mixed-culture systems 
      is helpful for monitoring the microflora in these cultures.
FAU - Sakai, Kenji
AU  - Sakai K
AD  - Department of Applied Chemistry, Faculty of Engineering, Oita University, 700 
      Dannoharu, Oita 870-1192, Japan. sakai@cc.oita-u.ac.jp
FAU - Ezaki, Yutaka
AU  - Ezaki Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Biosci Bioeng
JT  - Journal of bioscience and bioengineering
JID - 100888800
RN  - 0 (Bacteriocins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Industrial Waste)
RN  - 0 (Polyesters)
RN  - 0 (Polymers)
RN  - 0 (Sewage)
RN  - 0 (coagulin, Bacillus)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 459TN2L5F5 (poly(lactide))
SB  - IM
MH  - Bacteriocins/classification/genetics/*isolation & purification/*metabolism
MH  - Colony Count, Microbial/*methods
MH  - DNA, Bacterial/*analysis
MH  - Feasibility Studies
MH  - Fermentation
MH  - Food Industry
MH  - Food Microbiology
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Industrial Waste/*prevention & control
MH  - Lactic Acid/*metabolism
MH  - Polyesters
MH  - Polymers/metabolism
MH  - Sewage/microbiology
MH  - Species Specificity
EDAT- 2006/08/29 09:00
MHDA- 2006/10/07 09:00
CRDT- 2006/08/29 09:00
PHST- 2006/01/04 00:00 [received]
PHST- 2006/03/06 00:00 [accepted]
PHST- 2006/08/29 09:00 [pubmed]
PHST- 2006/10/07 09:00 [medline]
PHST- 2006/08/29 09:00 [entrez]
AID - S1389-1723(06)70610-7 [pii]
AID - 10.1263/jbb.101.457 [doi]
PST - ppublish
SO  - J Biosci Bioeng. 2006 Jun;101(6):457-63. doi: 10.1263/jbb.101.457.