PMID- 16935392
OWN - NLM
STAT- MEDLINE
DCOM- 20070123
LR  - 20191210
IS  - 0169-5002 (Print)
IS  - 0169-5002 (Linking)
VI  - 54
IP  - 2
DP  - 2006 Nov
TI  - Copy number gains on 5p15, 6p11-q11, 7p12, and 8q24 are rare in sputum cells of 
      individuals at high risk of lung cancer.
PG  - 169-76
AB  - Lung cancer specimens display recurrent copy number aberrations in distinguished 
      chromosomal regions as compared with normal lung cells. Such alterations have 
      been utilized in design of fluorescence in situ hybridization (FISH) probe sets 
      in attempts to improve the cytological diagnosis of lung cancer. One of such 
      probe sets, LAVysion, detects copy number changes in the centromeric region of 
      chromosome 6 (CEP6), and regions 5p15, 8q24, and 7p12, often gained in lung 
      cancer. METHODS: We evaluated the feasibility of the LAVysion multi-color probe 
      set in detection of individuals at high risk of lung cancer by applying the FISH 
      probe set on smears prepared of induced sputa obtained from 20 lung cancer 
      patients, 43 asbestos-exposed workers, 21 heavy tobacco smokers, and 15 healthy 
      never-smokers. The hybridized sputum smears were examined using fluorescence 
      microscopy and the number of signals in epithelial cells was examined throughout 
      the hybridized area. Additionally, we review here the previous studies using 
      LAVysion probe set. RESULTS: The FISH probe set was slightly more sensitive than 
      cytology alone in detecting lung cancer. No significant differences in copy 
      number gain were found between high-risk individuals and healthy never-smokers. 
      The proportions of individuals with copy number gains in sputa among the lung 
      cancer patients, asbestos-exposed workers, tobacco smokers, and never-smokers 
      were 50, 20, 12, and 27%, respectively, when three or more cells with a copy 
      number gain detected by at least two different probes was used as the cut-off 
      point. In comparison, the sensitivity of cytology in detecting lung cancer was 
      44%. In the lung cancer patients the number of abnormal cells by FISH correlated 
      well with the cytologic atypia class (Spearman rank correlation coefficient 0.77, 
      p<0.01). Using multivariant variance analysis, gains in CEP6, 5p15, 8q24 and 7p12 
      were not associated with smoking or asbestos exposure. CONCLUSIONS: FISH did not 
      significantly exceed the sensitivity of sputum cytology in finding lung cancers. 
      Significant differences were not found between sputa of asbestos-exposed 
      individuals, heavy-smokers and never-smokers. More sensitive methods are needed 
      for the follow-up of populations at high risk of contracting lung cancer.
FAU - Kettunen, Eeva
AU  - Kettunen E
AD  - Finnish Institute of Occupational Health, Centre of Expertise for Health and Work 
      Ability, Topeliuksenkatu 41 a A, FI-00250 Helsinki, Finland.
FAU - Salmenkivi, Kaisa
AU  - Salmenkivi K
FAU - Vuopala, Katri
AU  - Vuopala K
FAU - Toljamo, Tuula
AU  - Toljamo T
FAU - Kuosma, Eeva
AU  - Kuosma E
FAU - Norppa, Hannu
AU  - Norppa H
FAU - Knuutila, Sakari
AU  - Knuutila S
FAU - Kaleva, Simo
AU  - Kaleva S
FAU - Huuskonen, Matti S
AU  - Huuskonen MS
FAU - Anttila, Sisko
AU  - Anttila S
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060828
PL  - Ireland
TA  - Lung Cancer
JT  - Lung cancer (Amsterdam, Netherlands)
JID - 8800805
RN  - 1332-21-4 (Asbestos)
SB  - IM
MH  - Adenocarcinoma/*genetics
MH  - Adult
MH  - Aged
MH  - Asbestos/toxicity
MH  - Bronchi
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/diagnosis/*genetics
MH  - Male
MH  - Middle Aged
MH  - Risk
MH  - Sensitivity and Specificity
MH  - Smoking/adverse effects
MH  - Sputum/*cytology
EDAT- 2006/08/29 09:00
MHDA- 2007/01/24 09:00
CRDT- 2006/08/29 09:00
PHST- 2006/05/18 00:00 [received]
PHST- 2006/07/02 00:00 [revised]
PHST- 2006/07/15 00:00 [accepted]
PHST- 2006/08/29 09:00 [pubmed]
PHST- 2007/01/24 09:00 [medline]
PHST- 2006/08/29 09:00 [entrez]
AID - S0169-5002(06)00362-X [pii]
AID - 10.1016/j.lungcan.2006.07.009 [doi]
PST - ppublish
SO  - Lung Cancer. 2006 Nov;54(2):169-76. doi: 10.1016/j.lungcan.2006.07.009. Epub 2006 
      Aug 28.