PMID- 16935831
OWN - NLM
STAT- MEDLINE
DCOM- 20061019
LR  - 20131121
IS  - 1011-601X (Print)
IS  - 1011-601X (Linking)
VI  - 19
IP  - 3
DP  - 2006 Jul
TI  - Development and validation of RP-HPLC method for the analysis of metformin.
PG  - 231-5
AB  - The reversed-phase high-performance liquid chromatographic (RP-HPLC) method has 
      been developed to quantify metformin hydrochloride (MfCl) in raw material and 
      pharmaceutical formulations using C(18) analytical reverse-phase column. Diazepam 
      was used as an internal standard. Mobile phase consisted of methanol-water (30:70 
      v/v), pumped at a flow rate of 0.5 ml/min at ambient temperature and the 
      retention time was about 4.4 min with symmetrical peaks. (MfCl) was detected by 
      ultraviolet absorbance at 233 nm with no interference of commonly used 
      excipients. The method was linear over the concentration range 0.312-5 mug/mL (R2 
      = 0.9995). The limit of detection of metformin was 0.1 mug/mL and the limit of 
      quantitation was 0.3 mug/mL. The results obtained showed a good agreement with 
      the declared contents in case of pharmaceutical formulations. The proposed method 
      is rapid, accurate, economical and selective and it may be used for the 
      quantitative analysis of metformin in Neodipar tablets because of its sensitivity 
      and reproducibility.
FAU - Arayne, M Saeed
AU  - Arayne MS
AD  - Department of Chemistry, University of Karachi, Karachi-75270, Pakistan. 
      arayne@gawab.com
FAU - Sultana, Najma
AU  - Sultana N
FAU - Zuberi, M Hashim
AU  - Zuberi MH
LA  - eng
PT  - Journal Article
PL  - Pakistan
TA  - Pak J Pharm Sci
JT  - Pakistan journal of pharmaceutical sciences
JID - 9426356
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Pharmaceutical Solutions)
RN  - 0 (Tablets)
RN  - 9100L32L2N (Metformin)
SB  - IM
MH  - Calibration
MH  - Chemistry, Pharmaceutical
MH  - Chromatography, High Pressure Liquid
MH  - Hypoglycemic Agents/*analysis
MH  - Metformin/*analysis
MH  - Pharmaceutical Solutions
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Spectrophotometry, Ultraviolet
MH  - Tablets
EDAT- 2006/08/29 09:00
MHDA- 2006/10/20 09:00
CRDT- 2006/08/29 09:00
PHST- 2006/08/29 09:00 [pubmed]
PHST- 2006/10/20 09:00 [medline]
PHST- 2006/08/29 09:00 [entrez]
PST - ppublish
SO  - Pak J Pharm Sci. 2006 Jul;19(3):231-5.