PMID- 1693621
OWN - NLM
STAT- MEDLINE
DCOM- 19900713
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 110
IP  - 6
DP  - 1990 Jun
TI  - Subunit selectivity and epitope characterization of mAbs directed against the 
      GABAA/benzodiazepine receptor.
PG  - 2043-8
AB  - mAbs bd 17, bd 24, and bd 28 raised against bovine cerebral gamma-aminobutyric 
      acid (GABAA)/benzodiazepine receptors were analyzed for their ability to detect 
      each of 12 GABAA receptor subunits expressed in cultured mammalian cells. Results 
      showed that mAb bd 17 recognizes epitopes on both beta 2 and beta 3 subunits 
      while mAb bd 24 is selective for the alpha 1 subunit of human and bovine, but not 
      of rat origin. The latter antibody reacts with the rat alpha 1 subunit carrying 
      an engineered Leu at position four, documenting the first epitope mapping of a 
      GABAA receptor subunit-specific mAb. In contrast to mAbs bd 17 and bd 24, mAb bd 
      28 reacts with all GABAA receptor subunits tested but not with a glycine receptor 
      subunit, suggesting the presence of shared epitopes on subunits of GABA-gated 
      chloride channels.
FAU - Ewert, M
AU  - Ewert M
AD  - Center for Molecular Biology, University of Heidelberg, FRG.
FAU - Shivers, B D
AU  - Shivers BD
FAU - Luddens, H
AU  - Luddens H
FAU - Mohler, H
AU  - Mohler H
FAU - Seeburg, P H
AU  - Seeburg PH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Epitopes)
RN  - 0 (Receptors, GABA-A)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antibodies, Monoclonal/*immunology
MH  - Base Sequence
MH  - Cell Line, Transformed
MH  - Epitopes/*immunology
MH  - Humans
MH  - Immunohistochemistry
MH  - Kidney/cytology/embryology/*ultrastructure
MH  - Molecular Sequence Data
MH  - Receptors, GABA-A/analysis/*immunology/metabolism
PMC - PMC2116146
EDAT- 1990/06/01 00:00
MHDA- 1990/06/01 00:01
PMCR- 1990/12/01
CRDT- 1990/06/01 00:00
PHST- 1990/06/01 00:00 [pubmed]
PHST- 1990/06/01 00:01 [medline]
PHST- 1990/06/01 00:00 [entrez]
PHST- 1990/12/01 00:00 [pmc-release]
AID - 90277722 [pii]
AID - 10.1083/jcb.110.6.2043 [doi]
PST - ppublish
SO  - J Cell Biol. 1990 Jun;110(6):2043-8. doi: 10.1083/jcb.110.6.2043.