PMID- 16936436
OWN - NLM
STAT- MEDLINE
DCOM- 20060928
LR  - 20190819
IS  - 1346-9843 (Print)
IS  - 1346-9843 (Linking)
VI  - 70
IP  - 9
DP  - 2006 Sep
TI  - In vitro assessment of the effect of interleukin-1beta on angiogenic potential of 
      bone marrow cells.
PG  - 1195-9
AB  - BACKGROUND: Therapeutic angiogenesis for ischemic diseases has been successfully 
      induced by the implantation of autologous bone marrow cells (BMCs). It is 
      understood that interleukin (IL)-1beta increases remarkably in ischemic tissue 
      and has particular effects on angiogenesis. Thus, it is important to clarify how 
      IL-1beta would effect BMCs survival and angiogenic potential. METHODS AND 
      RESULTS: The effect of IL-1beta on BMCs survival, adhesion, and endothelial 
      differentiation, as well as the production of angiogenic growth factors, was 
      investigated using an in vitro assessment approach. BMCs were harvested from 
      Zucker obese rats and cultured at a density of 3x10(6) cells/ml with 5 ng/ml IL-1 
      beta (IL-1beta group) or without IL-1 beta (control group). Survival and adhesion 
      of BMCs were significantly increased in the IL-1beta group than in the control 
      group after 1, 3, and 7 days of culture (p<0.01). The release of vascular 
      endothelial growth factor in supernatant was also significantly higher in the 
      IL-1beta group than in the control group after 3 and 7 days of culture (p<0.01). 
      Furthermore, the number of differentiated endothelial cells derived from BMCs was 
      significantly higher in the IL-1beta group than in the control group after 7 days 
      of culture (p<0.01). CONCLUSIONS: These results suggest that IL-1beta has a 
      positive effect on the angiogenic potential of BMCs in vitro.
FAU - Qin, Shu-Lan
AU  - Qin SL
AD  - Department of Medical Bioregulation, Division of Cardiovascular Surgery, 
      Yamaguchi University School of Medicine, Japan.
FAU - Li, Tao-Sheng
AU  - Li TS
FAU - Takahashi, Masaya
AU  - Takahashi M
FAU - Hamano, Kimikazu
AU  - Hamano K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Circ J
JT  - Circulation journal : official journal of the Japanese Circulation Society
JID - 101137683
RN  - 0 (Interleukin-1)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology
MH  - Bone Marrow Transplantation
MH  - Cell Adhesion/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Interleukin-1/*pharmacology
MH  - Ischemia/therapy
MH  - Male
MH  - Neovascularization, Physiologic/*drug effects
MH  - Rats
MH  - Rats, Zucker
EDAT- 2006/08/29 09:00
MHDA- 2006/09/29 09:00
CRDT- 2006/08/29 09:00
PHST- 2006/08/29 09:00 [pubmed]
PHST- 2006/09/29 09:00 [medline]
PHST- 2006/08/29 09:00 [entrez]
AID - JST.JSTAGE/circj/70.1195 [pii]
AID - 10.1253/circj.70.1195 [doi]
PST - ppublish
SO  - Circ J. 2006 Sep;70(9):1195-9. doi: 10.1253/circj.70.1195.