PMID- 16936804
OWN - NLM
STAT- MEDLINE
DCOM- 20061012
LR  - 20131121
IS  - 0829-8211 (Print)
IS  - 0829-8211 (Linking)
VI  - 84
IP  - 3
DP  - 2006 Jun
TI  - Ontogenic changes in lactoferrin receptor and DMT1 in mouse small intestine: 
      implications for iron absorption during early life.
PG  - 337-44
AB  - It has been proposed that lactoferrin receptor (LfR) may be involved in 
      intestinal iron transport during early life. However, it is known that iron 
      homeostasis is regulated by divalent metal transporter 1 (DMT1; Nramp2/DCT1) in 
      the adult small intestine. To address the hypothesis that LfR may play a role as 
      an alternative iron transport pathway during early life, we used 
      immunohistochemistry (IHC) to examine the localization of mouse LfR (mLfR) and 
      DMT1. In addition to studying the localization and abundance of LfR and DMT1 on 
      the apical membrane, intestinal brush-border membrane vesicles (BBMV) were 
      isolated during the first 3 postnatal weeks (postnatal day (PD) 0, 5, 10, and 
      20). We found that mLfR is expressed in fetal mice as early as gestational days 
      (GD) 13.5, 15.5, and 18.5. A 34 kD band for mLfR was detected at PD 0 through PD 
      20 in total intestine homogenate. However, mLfR protein did not appear in the 
      BBMV preparations until PD 5 and was highly expressed at PD 10. By IHC, DMT1 
      protein was minimally observed at PD 0 and PD 5, but by PD 10 DMT1 was 
      predominantly localized in the apical membrane of the maturing intestine. BBMV 
      fractionation revealed 50-120 kD protein bands for DMT1. In these BBMV 
      preparations, the apical-membrane-associated 120 kD band for DMT1 increased in 
      abundance with age. However, in the corresponding total homogenates, only the 
      deglycosylated form of DMT1 (50 kD) was identified. These results indicate that 
      DMT1 is mislocalized during late gestation, minimally expressed during early 
      life, and predominantly expressed in its deglycosylated form until PD 20. The 
      immunolocalization and abundant protein expression of mLfR suggest that accrual 
      of iron from Lf may be the principal iron uptake pathway at this age. In 
      conclusion, our findings support the notion that until the development-dependent 
      expression of DMT1 in the intestine is induced, mLfR may serve as an alternative 
      iron uptake pathway.
FAU - Lopez, Veronica
AU  - Lopez V
AD  - Department of Nutrition, University of California, 3217C Meyer Hall, One Shields 
      Ave, Davis, CA 95616, USA.
FAU - Suzuki, Yasushi A
AU  - Suzuki YA
FAU - Lonnerdal, Bo
AU  - Lonnerdal B
LA  - eng
GR  - HD-043240/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - Canada
TA  - Biochem Cell Biol
JT  - Biochemistry and cell biology = Biochimie et biologie cellulaire
JID - 8606068
RN  - 0 (Cation Transport Proteins)
RN  - 0 (Iron-Binding Proteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (lactoferrin receptors)
RN  - 0 (solute carrier family 11- (proton-coupled divalent metal ion transporters), 
      member 2)
RN  - E1UOL152H7 (Iron)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cation Transport Proteins/*metabolism
MH  - Female
MH  - Intestine, Small/cytology/*metabolism
MH  - Iron/*metabolism
MH  - Iron-Binding Proteins/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Receptors, Cell Surface/*metabolism
EDAT- 2006/08/29 09:00
MHDA- 2006/10/13 09:00
CRDT- 2006/08/29 09:00
PHST- 2006/08/29 09:00 [pubmed]
PHST- 2006/10/13 09:00 [medline]
PHST- 2006/08/29 09:00 [entrez]
AID - o06-059 [pii]
AID - 10.1139/o06-059 [doi]
PST - ppublish
SO  - Biochem Cell Biol. 2006 Jun;84(3):337-44. doi: 10.1139/o06-059.