PMID- 16936849
OWN - NLM
STAT- MEDLINE
DCOM- 20061114
LR  - 20161020
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 49
IP  - 6
DP  - 2006 Jun
TI  - Characterization of a maize isochromosome 8S*8S.
PG  - 700-6
AB  - An isochromosome was found in the maize HiII Parent B line during somatic 
      karyotyping with a multiprobe fluorescence in situ hybridization (FISH) system. 
      Cytological analyses showed that it pairs with the short arm of chromosome 8 
      during the pachytene stage of meiosis. The chromosome 8 short arm origin of this 
      isochromosome was also confirmed by FISH at mitotic metaphase. Knob 
      heterochromatin signals were present at the short arms of chromosome 8 when 
      subjected to prolonged exposure and also observed at both ends of the 
      isochromosome. This isochromosome can be a univalent or a trivalent by pairing 
      with the normal chromosome 8 short arms during meiosis. At anaphase and 
      telophase, the isochromosome lagged behind other chromosomes. It had a 
      transmission rate of 17%-20% from both male and female gametes. One plant 
      homozygous for the isochromosome contained 2 isochromosomes that differed in the 
      quantity of their CentC centromere repeat sequence. Both variations of the 
      isochromosome were transmitted to the next generation. Because the 2 
      isochromosomes should be identical by descent, these observations document a 
      radical change in copy number of the centromere repeat array within 1 generation. 
      Plants with 1 isochromosome were not normal as compared with the original HiII 
      Parent B plants. Those that contained a pair of this isochromosome (6 total 
      copies of 8S) were even more abnormal and had reduced fertility. The results 
      indicate the ability of the somatic karyotyping system to recognize and 
      characterize chromosomal aberrations.
FAU - Yu, Weichang
AU  - Yu W
AD  - Division of Biological Sciences, University of Missouri-Columbia, 65211, USA.
FAU - Han, Fangpu
AU  - Han F
FAU - Kato, Akio
AU  - Kato A
FAU - Birchler, James A
AU  - Birchler JA
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
SB  - IM
MH  - Centromere
MH  - Chromosome Aberrations
MH  - Chromosomes, Plant
MH  - Gene Duplication
MH  - Genome, Plant
MH  - Homozygote
MH  - In Situ Hybridization, Fluorescence
MH  - Inheritance Patterns
MH  - *Isochromosomes
MH  - Karyotyping
MH  - Meiosis
MH  - Phenotype
MH  - Zea mays/*genetics
EDAT- 2006/08/29 09:00
MHDA- 2006/11/15 09:00
CRDT- 2006/08/29 09:00
PHST- 2006/08/29 09:00 [pubmed]
PHST- 2006/11/15 09:00 [medline]
PHST- 2006/08/29 09:00 [entrez]
AID - g06-019 [pii]
AID - 10.1139/g06-019 [doi]
PST - ppublish
SO  - Genome. 2006 Jun;49(6):700-6. doi: 10.1139/g06-019.