PMID- 169380
OWN - NLM
STAT- MEDLINE
DCOM- 19751120
LR  - 20031114
IS  - 0027-8874 (Print)
IS  - 0027-8874 (Linking)
VI  - 55
IP  - 2
DP  - 1975 Aug
TI  - Mouse mammary tumor virus polypeptide precursors in intracytoplasmic A particles.
PG  - 493-6
AB  - Intracytoplasmic A particles were analyzed by immunodiffusion and sodium dodecyl 
      sulfate--polyacrylamide gel electrophoresis (SDS--PAGE) before and after 
      enzymatic cleavage with trypsin. A common antigen in A particles was detected by 
      antisera prepared against purified intracytoplasmic A particles, purified mouse 
      mammary tumor virus (MMTV), and a purified MMTV core polypeptide (p28). Despite 
      this correlation, no SDS--polyacrylamide band migrating at p28 was observed in 
      purified intracytoplasmic A particles. However, after incubation with trypsin, A 
      particles subjected to SDS--PAGE produced only two polypeptide bands. They were 
      observed at p28 and p15-10. Ouchterlony analysis of the trypsin-cleaved A 
      particles revealed no alteration in the antigenicity of the particles. These 
      results suggested that some structural components of intracytoplasmic A particles 
      are polypeptide precursors of MMTV core proteins.
FAU - Smith, G H
AU  - Smith GH
FAU - Lee, B K
AU  - Lee BK
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Natl Cancer Inst
JT  - Journal of the National Cancer Institute
JID - 7503089
RN  - 0 (Antigens)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Antigens
MH  - Cells, Cultured
MH  - Cross Reactions
MH  - Female
MH  - Inclusion Bodies, Viral/analysis/immunology/*metabolism
MH  - Mammary Tumor Virus, Mouse/*growth & development/immunology
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Viral Proteins/analysis/immunology/*metabolism
EDAT- 1975/08/01 00:00
MHDA- 1975/08/01 00:01
CRDT- 1975/08/01 00:00
PHST- 1975/08/01 00:00 [pubmed]
PHST- 1975/08/01 00:01 [medline]
PHST- 1975/08/01 00:00 [entrez]
PST - ppublish
SO  - J Natl Cancer Inst. 1975 Aug;55(2):493-6.