PMID- 16938306
OWN - NLM
STAT- MEDLINE
DCOM- 20061215
LR  - 20131121
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1133
IP  - 1-2
DP  - 2006 Nov 10
TI  - Control of the keto-enol tautomerism of chlortetracycline for its straightforward 
      quantitation in pig tissues by liquid chromatography-electrospray ionization 
      tandem mass spectrometry.
PG  - 135-41
AB  - Chlortetracycline (CTC) is a member of the group of the tetracycline antibiotics 
      used in human and veterinary medicine. Its reliable measurement in edible tissues 
      is hampered by the fact that CTC is subjected to keto-enol tautomerism resulting 
      in its keto- and enol-form. Also, it undergoes epimerization to form its 
      4-epimer. In this paper, we present an LC-electrospray ionization (ESI)-MS/MS 
      method to analyze the CTC content in pig tissues, with the originality that it 
      apports a solution to overcome the possible interference on the measurement of 
      the keto-enol tautomerism equilibrium, which is influenced by several factors. 
      Therefore, the minced tissue samples were incubated at 56 degrees C, forcing the 
      equilibrium in favour of the CTC keto-form. The CTC keto-tautomer, as a measure 
      of the total CTC content, was then quantified by LC-ESI-MS/MS, after further 
      extraction of the tissue samples, consisting of a liquid extraction with sodium 
      succinate solution (pH 4.0), followed by protein removal with trichloroacetic 
      acid and paper filtration, and finally solid-phase clean-up on an 
      hydrophilic-lipophilic balance (HLB) polymeric reversed-phase column. The method 
      could be validated for pig muscle, liver and kidney tissues--for which maximum 
      residue limits (MRLs) have been fixed by the European Union--with respect to 
      linearity, trueness and precision, in accordance with the European guidelines.
FAU - Cherlet, Marc
AU  - Cherlet M
AD  - Ghent University, Faculty of Veterinary Medicine, Department of Pharmacology, 
      Pharmacy and Toxicology, Salisburylaan 133, B-9820 Merelbeke, Belgium. 
      mcherlet@yahoo.com
FAU - De Backer, Patrick
AU  - De Backer P
FAU - Croubels, Siska
AU  - Croubels S
LA  - eng
PT  - Journal Article
DEP - 20060830
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - WCK1KIQ23Q (Chlortetracycline)
SB  - IM
MH  - Animals
MH  - Chlortetracycline/analysis/*chemistry
MH  - Chromatography, Liquid/*methods
MH  - Reproducibility of Results
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Stereoisomerism
MH  - Swine
EDAT- 2006/08/30 09:00
MHDA- 2006/12/16 09:00
CRDT- 2006/08/30 09:00
PHST- 2006/05/03 00:00 [received]
PHST- 2006/07/18 00:00 [revised]
PHST- 2006/08/07 00:00 [accepted]
PHST- 2006/08/30 09:00 [pubmed]
PHST- 2006/12/16 09:00 [medline]
PHST- 2006/08/30 09:00 [entrez]
AID - S0021-9673(06)01513-5 [pii]
AID - 10.1016/j.chroma.2006.08.008 [doi]
PST - ppublish
SO  - J Chromatogr A. 2006 Nov 10;1133(1-2):135-41. doi: 10.1016/j.chroma.2006.08.008. 
      Epub 2006 Aug 30.