PMID- 16938516
OWN - NLM
STAT- MEDLINE
DCOM- 20061024
LR  - 20161124
IS  - 0046-8177 (Print)
IS  - 0046-8177 (Linking)
VI  - 37
IP  - 9
DP  - 2006 Sep
TI  - The value of MDM2 and CDK4 amplification levels using real-time polymerase chain 
      reaction for the differential diagnosis of liposarcomas and their histologic 
      mimickers.
PG  - 1123-9
AB  - Atypical lipomatous tumor/well-differentiated liposarcoma (ALT/WDL) and 
      dedifferentiated liposarcoma (DDL) are reported to have murine double-minute type 
      2 (MDM2) and cyclin-dependent kinase 4 (CDK4) amplification as a characteristic 
      genetic alteration. To evaluate the diagnostic utility of this gene abnormality, 
      we analyzed 19 liposarcomas, 21 malignant fibrous histiocytomas, 3 
      leiomyosarcomas, 5 malignant peripheral nerve sheath tumors, 23 lipomas, and 28 
      nonneoplastic fat tissues using real-time polymerase chain reaction (PCR) and 
      fluorescence in situ hybridization (FISH). In real-time PCR, all ALT/WDLs and 
      DDLs had both MDM2 and CDK4 amplifications. The amplification levels in ALT/WDLs 
      and DDLs were significantly higher than those in the other sarcomas, lipomas, and 
      nonneoplastic fat tissues (P < .05); however, those in the other sarcomas and 
      lipomas were not significantly higher than those in nonneoplastic tissues. In 
      FISH, all ALT/WDLs and DDLs had both MDM2 and CDK4 amplifications, and all of the 
      myxoid/round cell liposarcomas, leiomyosarcomas, malignant peripheral nerve 
      sheath tumors, and all but one of the malignant fibrous histiocytomas did not 
      have the amplifications. In this study, MDM2 and CDK4 amplifications were 
      confirmed in ALT/WDLs and DDLs, and the amplification levels were significantly 
      higher than those in the other tumors. An analysis of MDM2 and CDK4 amplification 
      using real-time PCR, as well as FISH, is useful for the differential diagnosis of 
      liposarcomas and their histologic mimickers.
FAU - Shimada, Shio
AU  - Shimada S
AD  - Department of Pathology, Saitama Medical University School of Medicine, Saitama 
      350-0495, Japan. shio@saitama-med.ac.jp
FAU - Ishizawa, Takashi
AU  - Ishizawa T
FAU - Ishizawa, Keisuke
AU  - Ishizawa K
FAU - Matsumura, Tadaki
AU  - Matsumura T
FAU - Hasegawa, Tadashi
AU  - Hasegawa T
FAU - Hirose, Takanori
AU  - Hirose T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20060707
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 0 (Biomarkers, Tumor)
RN  - EC 2.3.2.27 (MDM2 protein, human)
RN  - EC 2.3.2.27 (Proto-Oncogene Proteins c-mdm2)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinase 4)
SB  - IM
MH  - Adipose Tissue/pathology/physiology
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/*genetics
MH  - Cyclin-Dependent Kinase 4/*genetics
MH  - Diagnosis, Differential
MH  - Female
MH  - Gene Amplification
MH  - Histiocytoma, Malignant Fibrous/diagnosis/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Leiomyosarcoma/diagnosis/genetics
MH  - Lipoma/diagnosis/genetics
MH  - Liposarcoma/*diagnosis/*genetics
MH  - Male
MH  - Middle Aged
MH  - Nerve Sheath Neoplasms/diagnosis/genetics
MH  - Proto-Oncogene Proteins c-mdm2/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2006/08/30 09:00
MHDA- 2006/10/25 09:00
CRDT- 2006/08/30 09:00
PHST- 2005/11/23 00:00 [received]
PHST- 2006/04/10 00:00 [revised]
PHST- 2006/04/11 00:00 [accepted]
PHST- 2006/08/30 09:00 [pubmed]
PHST- 2006/10/25 09:00 [medline]
PHST- 2006/08/30 09:00 [entrez]
AID - S0046-8177(06)00223-1 [pii]
AID - 10.1016/j.humpath.2006.04.010 [doi]
PST - ppublish
SO  - Hum Pathol. 2006 Sep;37(9):1123-9. doi: 10.1016/j.humpath.2006.04.010. Epub 2006 
      Jul 7.