PMID- 16938572
OWN - NLM
STAT- MEDLINE
DCOM- 20061030
LR  - 20091119
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 169
IP  - 2
DP  - 2006 Sep
TI  - Identification of recurrent chromosomal breakpoints in multiple myeloma with 
      complex karyotypes by combined G-banding, spectral karyotyping, and fluorescence 
      in situ hybridization analyses.
PG  - 143-9
AB  - The description of novel chromosomal aberrations in multiple myeloma (MM) remains 
      necessary to fully understand the pathogenesis of this heterogeneous disease. 
      Therefore, we have used spectral karyotyping (SKY) and fluorescence in situ 
      hybridization (FISH) with locus-specific probes to characterize the chromosomal 
      abnormalities in 11 MM cases in which G-banding revealed a complex karyotype. SKY 
      refined G-banding karyotypes in all cases. Recurrent breakpoints involved bands 
      Xp11, 8q24, 11q13, 12q13, 13q21, and 14q32. In addition, combined SKY and FISH 
      analyses permitted us to identify a subset of patients harboring 22q11.2 
      rearrangements not involving the IGL locus. This finding suggests the presence of 
      other gene(s) in band 22q11 that might be implicated in MM pathogenesis. 
      Moreover, band 1p13 was identified as a novel partner of immunoglobulin (IG) 
      translocations in MM. Finally, using interphase FISH, we have detected 
      interstitial deletions in 13q14 and 17p13, as well as cryptic translocations 
      affecting IGH, which were neither detected by G-banding nor by SKY. The results 
      of the present study suggest the existence of hitherto unknown nonrandom 
      chromosomal changes that may play a role in the pathogenesis of MM. Our findings 
      underline the importance of the combination of banding, SKY, and FISH analyses to 
      increase the accuracy of karyotype interpretation in plasma cell neoplasias.
CI  - Copyright 2006 Elsevier Inc.
FAU - Saez, Borja
AU  - Saez B
AD  - Department of Genetics, University of Navarra, Irunlarrea s/n, Pamplona, Navarra 
      31008, Spain. bsaezoch@alumni.unav.es
FAU - Martin-Subero, Jose I
AU  - Martin-Subero JI
FAU - Largo, Cristina
AU  - Largo C
FAU - Martin, Maria C
AU  - Martin MC
FAU - Odero, Maria D
AU  - Odero MD
FAU - Prosper, Felipe
AU  - Prosper F
FAU - Siebert, Reiner
AU  - Siebert R
FAU - Calasanz, Maria J
AU  - Calasanz MJ
FAU - Cigudosa, Juan C
AU  - Cigudosa JC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Cyclin D)
RN  - 0 (Cyclins)
SB  - IM
MH  - *Chromosome Aberrations
MH  - *Chromosome Banding
MH  - Chromosomes, Human, Pair 13
MH  - Cyclin D
MH  - Cyclins/genetics
MH  - Genes, Immunoglobulin
MH  - Genes, myc
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Multiple Myeloma/*genetics
MH  - *Spectral Karyotyping
EDAT- 2006/08/30 09:00
MHDA- 2006/10/31 09:00
CRDT- 2006/08/30 09:00
PHST- 2006/01/30 00:00 [received]
PHST- 2006/04/07 00:00 [revised]
PHST- 2006/04/11 00:00 [accepted]
PHST- 2006/08/30 09:00 [pubmed]
PHST- 2006/10/31 09:00 [medline]
PHST- 2006/08/30 09:00 [entrez]
AID - S0165-4608(06)00258-5 [pii]
AID - 10.1016/j.cancergencyto.2006.04.008 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2006 Sep;169(2):143-9. doi: 
      10.1016/j.cancergencyto.2006.04.008.