PMID- 16940288
OWN - NLM
STAT- MEDLINE
DCOM- 20070612
LR  - 20131121
IS  - 1470-1626 (Print)
IS  - 1470-1626 (Linking)
VI  - 132
IP  - 3
DP  - 2006 Sep
TI  - 17beta-estradiol induces Akt-1 through estrogen receptor-beta in the frog (Rana 
      esculenta) male germ cells.
PG  - 477-84
AB  - Several lines of evidence support the key role of estrogens in male fertility. 
      Here, we investigate the regulation of the serine/threonine kinase Akt-1 in the 
      frog (Rana esculenta) testis during the annual sexual cycle and, whether 
      17beta-estradiol (E2) exerts a role in the Akt-1 activity. Akt-1 has been shown 
      to be the mediator of growth factor-dependent cell proliferation, survival, and 
      metabolism in a variety of cell types. First, we demonstrate by 
      immunohistochemistry, the presence of estrogen receptor-beta (ERbeta), and Akt-1 
      in the spermatogonia (SPG), spermatocytes (SPC), and spermatids (SPT). 
      Western-blot analysis revealed that ERbeta isoform (molecular weight 55 kDa) was 
      highly expressed in May (reproductive period) with respect to January and 
      November (winter stasis); in parallel, Akt-1 (molecular weight 60 kDa) is highly 
      phosphorylated (Ser-473) during the period of active spermatogenesis (May) 
      compared with the winter stasis (January and November). In addition, in vitro 
      experiments demonstrate that E2 treatment induces the activation of Akt-1, and 
      this effect is counteracted by the anti-estrogen ICI 182-780. In conclusion, our 
      data show that E2 induces Akt-1 phosphorylation (Ser-473) possibly via ERbeta in 
      frog (R. esculenta) male germ cells.
FAU - Stabile, Veronica
AU  - Stabile V
AD  - Dipartimento di Medicina Sperimentale, II Universita di Napoli, Via 
      Costantinopoli, 16, 80138 Napoli, Naples, Italy.
FAU - Russo, Maria
AU  - Russo M
FAU - Chieffi, Paolo
AU  - Chieffi P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Reproduction
JT  - Reproduction (Cambridge, England)
JID - 100966036
RN  - 0 (Estrogen Receptor beta)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Animals
MH  - Blotting, Western/methods
MH  - Estradiol/*pharmacology
MH  - Estrogen Receptor beta/*metabolism
MH  - Immunohistochemistry/methods
MH  - Male
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - Rana esculenta/*metabolism
MH  - *Seasons
MH  - Spermatogenesis
MH  - Spermatozoa/*metabolism
EDAT- 2006/08/31 09:00
MHDA- 2007/06/15 09:00
CRDT- 2006/08/31 09:00
PHST- 2006/08/31 09:00 [pubmed]
PHST- 2007/06/15 09:00 [medline]
PHST- 2006/08/31 09:00 [entrez]
AID - 132/3/477 [pii]
AID - 10.1530/rep.1.01107 [doi]
PST - ppublish
SO  - Reproduction. 2006 Sep;132(3):477-84. doi: 10.1530/rep.1.01107.