PMID- 1694437
OWN - NLM
STAT- MEDLINE
DCOM- 19900806
LR  - 20180215
IS  - 1015-2008 (Print)
IS  - 1015-2008 (Linking)
VI  - 58
IP  - 2
DP  - 1990
TI  - Clinical flow cytometric reticulocyte analysis.
PG  - 99-106
AB  - The flow cytometric (FCM) analysis of reticulocytes in a clinical laboratory can 
      be accomplished using acridine orange (AO), thiazole orange (TO), auramine O, 
      thioflavin T, pyronin Y, dimethyl-oxacarbocyanine or transferrin receptor assays. 
      AO and TO are vital stains, show good correlation with microscopic reticulocyte 
      determinations and, when compared to each other, give an excellent correlation. 
      The coefficient of variation is below 5% and batch analysis on blood samples 
      stored for 96 h further reduces manpower needs. Finally we have used the mean 
      fluorescent intensity of TO as a reticulocyte maturity index (RMI). In bone 
      marrow transplant patients, the RMI has been the earliest indicator of marrow 
      engraphment. Using the RMI we have been able to define three patterns of 
      engraphment: early, delayed and failed. Although a variety of standards will be 
      required, the clinical FCM reticulocyte analysis promises to be the preferred and 
      accepted method in the clinical laboratory.
FAU - Davis, B H
AU  - Davis BH
AD  - Department of Pathology, Dartmouth Hitchcock Medical Center, Hanover, N.H.
FAU - Bigelow, N C
AU  - Bigelow NC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Pathobiology
JT  - Pathobiology : journal of immunopathology, molecular and cellular biology
JID - 9007504
RN  - 0 (Benzothiazoles)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Quinolines)
RN  - 0 (Thiazoles)
RN  - 107091-89-4 (thiazole orange)
RN  - 63231-63-0 (RNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Benzothiazoles
MH  - Blood Cell Count
MH  - Cell Differentiation
MH  - Flow Cytometry
MH  - Fluorescent Dyes
MH  - Humans
MH  - Quinolines
MH  - RNA/metabolism
MH  - *Reticulocytes/cytology
MH  - Thiazoles
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - 10.1159/000163571 [doi]
PST - ppublish
SO  - Pathobiology. 1990;58(2):99-106. doi: 10.1159/000163571.