PMID- 16946269
OWN - NLM
STAT- MEDLINE
DCOM- 20061026
LR  - 20201109
IS  - 1350-0872 (Print)
IS  - 1350-0872 (Linking)
VI  - 152
IP  - Pt 9
DP  - 2006 Sep
TI  - Regulation of the expression of whiB1 in Mycobacterium tuberculosis: role of cAMP 
      receptor protein.
PG  - 2749-2756
LID - 10.1099/mic.0.28924-0 [doi]
AB  - The wbl (whiB-like) genes encode putative transcription factors unique to 
      actinomycetes. This study characterized the promoter element of one of the seven 
      wbl genes of Mycobacterium tuberculosis, whiB1 (Rv3219c). The results reveal that 
      whiB1 is transcribed by a class I-type cAMP receptor protein (CRP)-dependent 
      promoter, harbouring a CRP-binding site positioned at -58.5 with respect to its 
      transcription start point. In vivo promoter activity analysis and electrophoretic 
      mobility shift assays suggest that the expression of whiB1 is indeed regulated by 
      cAMP-dependent binding of CRP(M) (encoded by the M. tuberculosis gene Rv3676) to 
      the whiB1 5' untranslated region (5'UTR). beta-Galactosidase gene fusion analysis 
      revealed induction of the whiB1 promoter in M. tuberculosis on addition of 
      exogenous dibutyric cAMP (a diffusible cAMP analogue) only when an intact 
      CRP-binding site was present. These results indicate that M. tuberculosis whiB1 
      transcription is regulated in part by cAMP levels via direct binding of 
      cAMP-activated CRP(M) to a consensus CRP-binding site in the whiB1 5'UTR.
FAU - Agarwal, Nisheeth
AU  - Agarwal N
AD  - Department of Medicine, Johns Hopkins University, CRB2, Rm 1.08, 1550 Orleans 
      Street, Baltimore, MD 21231-1044, USA.
FAU - Raghunand, Tirumalai R
AU  - Raghunand TR
AD  - Department of Medicine, Johns Hopkins University, CRB2, Rm 1.08, 1550 Orleans 
      Street, Baltimore, MD 21231-1044, USA.
FAU - Bishai, William R
AU  - Bishai WR
AD  - Department of Medicine, Johns Hopkins University, CRB2, Rm 1.08, 1550 Orleans 
      Street, Baltimore, MD 21231-1044, USA.
LA  - eng
GR  - AI36973/AI/NIAID NIH HHS/United States
GR  - AI37856/AI/NIAID NIH HHS/United States
GR  - AI43846/AI/NIAID NIH HHS/United States
GR  - AI51668/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - England
TA  - Microbiology (Reading)
JT  - Microbiology (Reading, England)
JID - 9430468
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Cyclic AMP Receptor Protein)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 63X7MBT2LQ (Bucladesine)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - 5' Untranslated Regions
MH  - Bacterial Proteins/*genetics
MH  - Base Sequence
MH  - Binding Sites
MH  - Bucladesine/pharmacology
MH  - Cyclic AMP Receptor Protein/genetics/*metabolism
MH  - *Gene Expression Regulation, Bacterial
MH  - Genes, Bacterial
MH  - Molecular Sequence Data
MH  - Mycobacterium tuberculosis/*genetics
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - Recombinant Fusion Proteins/metabolism
MH  - Transcription, Genetic
MH  - beta-Galactosidase/metabolism
EDAT- 2006/09/02 09:00
MHDA- 2006/10/27 09:00
CRDT- 2006/09/02 09:00
PHST- 2006/09/02 09:00 [pubmed]
PHST- 2006/10/27 09:00 [medline]
PHST- 2006/09/02 09:00 [entrez]
AID - 10.1099/mic.0.28924-0 [doi]
PST - ppublish
SO  - Microbiology (Reading). 2006 Sep;152(Pt 9):2749-2756. doi: 10.1099/mic.0.28924-0.