PMID- 16948491
OWN - NLM
STAT- MEDLINE
DCOM- 20061012
LR  - 20151119
IS  - 1478-1581 (Print)
IS  - 1478-1581 (Linking)
VI  - 153
IP  - 4
DP  - 2006 Aug
TI  - Integration of microfluidics with biomedical infrared spectroscopy for analytical 
      and diagnostic metabolic profiling.
PG  - 74-80
AB  - We describe how infrared spectroscopy of dry films (IRDF) can provide diagnostic 
      information, and how we expect integration with laminar fluid diffusion interface 
      (LFDI) sample pre-processing to generate new analytical and diagnostic tests. 
      LFDI pre-processing provides sample clean-up and analyte separation. The 
      sensitivity of IRDF to certain analytes is enhanced through the depletion of 
      sample constituents that otherwise obscure relevant spectral features, permitting 
      the deposition of films with larger sample volumes and, hence, of greater 
      effective optical pathlength for the targeted analytes. An integrated LFDI-IRDF 
      technology holds promise both as a method for rapid point-of-care quantitative 
      analysis of biological fluids and as the engine of discovery for a wide range of 
      novel diagnostic methods based upon metabolic profiling. In particular, 
      successful integration will provide a versatile and cost effective technology 
      platform that will allow for the accurate quantification of low-concentration 
      analytes that are otherwise inaccessible and will provide the basis for 
      diagnostic and prognostic methods that would otherwise be impossible. The 
      specific question addressed by the proof-of-concept study summarised here is 
      whether the spectra of LFDI processed samples can provide analytical methods that 
      are more accurate than otherwise possible without LFDI pre-processing. The 
      enrichment of serum creatinine is accomplished, with subsequent enhancement of 
      its spectral contribution permitting quantification of this clinically important 
      analyte beyond that achievable with no pre-processing. Finally, to illustrate the 
      potential in diagnostic applications, two recently initiated studies are 
      outlined, one involving chronic kidney disease and the other for chronic and 
      acute coronary artery disease.
FAU - Mansfield, C D
AU  - Mansfield CD
AD  - Institute for Biodiagnostics, National Research Council Canada, 435 Elice Avenue, 
      Winnipeg, Manitoba R3B 1Y6, Canada. Colin.Mansfield@physics.org
FAU - Man, A
AU  - Man A
FAU - Shaw, R A
AU  - Shaw RA
LA  - eng
PT  - Journal Article
PL  - England
TA  - IEE Proc Nanobiotechnol
JT  - IEE proceedings. Nanobiotechnology
JID - 101188789
RN  - 0 (Biomarkers)
SB  - IM
MH  - Biomarkers/*blood
MH  - Biomedical Engineering/instrumentation/*methods
MH  - Blood Chemical Analysis/instrumentation/*methods
MH  - Diagnosis, Computer-Assisted/*methods
MH  - Gene Expression Profiling/instrumentation/*methods
MH  - Humans
MH  - Microfluidic Analytical Techniques/instrumentation/*methods
MH  - Spectrophotometry, Infrared/instrumentation/*methods
MH  - Systems Integration
EDAT- 2006/09/05 09:00
MHDA- 2006/10/13 09:00
CRDT- 2006/09/05 09:00
PHST- 2006/09/05 09:00 [pubmed]
PHST- 2006/10/13 09:00 [medline]
PHST- 2006/09/05 09:00 [entrez]
AID - 10.1049/ip-nbt:20050028 [doi]
PST - ppublish
SO  - IEE Proc Nanobiotechnol. 2006 Aug;153(4):74-80. doi: 10.1049/ip-nbt:20050028.